Yang Wang, Jianhong Liu, Haizhen Yu, Shizhen Li, Wenyan Wang
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引用次数: 0
Abstract
Our aim was to ascertain the clinical implications of microRNA-487b-3p (miR-487b-3p) in breast cancer. MiR-487b-3p level was measured in breast cancer tissues (n = 102) and adjacent noncancerous tissues (n = 102) using quantitative real-time polymerase chain reaction (qRT-PCR). Kaplan-Meier method and Cox regression analysis were conducted for evaluating the clinical performance of miR-487b-3p. Cell counting kit-8 (CCK-8) and Transwell assay were utilized for cellular functions. Bioinformatics tools and luciferase reporter assay were performed for the target predication and confirmation. MiR-487b-3p was remarkably diminished in breast cancer tissues. Aberrant miR-487b-3p was tightly in relation to Ki67 (p = 0.003), human epidermal growth factor receptor (HER2) status (p = 0.025), tumor node metastasis (TNM) stage (p = 0.028) and lymph node metastasis (p = 0.002). Moreover, a better progression free survival rate was found in patients expressing high miR-487b-3p (n = 51, p < 0.001) than those with low expression (n = 51). Additionally, miR-487b-3p was an independent predictor for the prognosis of breast cancer (p < 0.001; HR = 2.971; 95% CI = 1.721–5.131). Enforced miR-487b-3p expression obviously restrained cell activities and epithelial-mesenchymal transition (EMT), while apposite effects were observed posttransfection with miR-487b-3p inhibitor (p < 0.001). Notably, low-density lipoprotein receptor-related protein 6 (LRP6) was a potential target gene of miR-487b-3p, an adverse relevance of which was discovered (r = −0.5811; p < 0.001). MiR-487b-3p was an underlying indicator for the prognosis in breast cancer. It impacted on cell activities and EMT via regulating LRP6 in breast cancer development.
期刊介绍：
The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.
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