Maltoheptaose-based block copolymer nanoparticles containing magnesium phthalocyanine: Formulation, photodynamic activity, release kinetic, toxicity, and biodistribution

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-05-09 DOI:10.1016/j.molliq.2025.127737

Lia Mallmann Mattiazzi , Edileuza Pinto Teixeira , Brenda Bopp Baptista , Felix Alexandre Antunes Soares , Marcell Valandro Soares , Cristiane de Bona da Silva , Hong Li , Sami Halila , Marcos Antonio Villetti , Redouane Borsali

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引用次数: 0

Abstract

Photodynamic therapy (PDT) has gained prominence in treating both neoplastic and non-neoplastic diseases due to the advent of new third-generation photosensitizers. This study focused on the creation of glyconanoparticles based on block copolymers (maltoheptaose-b-polystyrene and maltoheptaose-b-polymethylmethacrylate), encapsulating the photosensitizer magnesium phthalocyanine (MgPc), produced through a self-assembly process in a selected solvent mixture. The physicochemical characteristics of the polymeric micelles were analyzed, including their photodynamic efficacy, release kinetic profile, in vivo toxicity, impact on larval development, and biodistribution in Caenorhabditis elegans nematodes. The glyconanoparticles exhibited favorable physicochemical properties, with entrapment efficiency exceeding 96% and an estimated shelf life of three years. Moreover, both MH-b-PS@MgPc and MH-b-PMMA@MgPc nanocarriers demonstrated suitable singlet oxygen quantum yield values for PDT application, closely mirroring those of free MgPc. The drug release behavior highlighted the significance of the hydrophobic block’s nature within the core of polymeric micelles in influencing the sustained-release phase, indicating a preferential interaction of MgPc with polymethylmethacrylate over polystyrene. Additionally, at low concentrations, MH-b-PS@MgPc and MH-b-PMMA@MgPc were found to be non-lethal to C. elegans, and post-oral ingestion, they predominantly accumulated in the intestinal surrounding cells. Our findings underscore the potential of glyconanoparticles containing MgPc in advancing third-generation photosensitizers for PDT applications.

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含有酞菁镁的麦芽糖醛酸基嵌段共聚物纳米颗粒：配方、光动力学活性、释放动力学、毒性和生物分布

由于第三代光敏剂的出现，光动力疗法（PDT）在治疗肿瘤和非肿瘤疾病方面都取得了突出的成就。本研究的重点是基于嵌段共聚物（麦芽糖heptaose-b-聚苯乙烯和麦芽糖heptaose-b-聚甲基丙烯酸甲酯）的glyconanopparticles的创建，封装光敏剂酞菁镁（MgPc），通过在选定的溶剂混合物中自组装工艺生产。分析了聚合物胶束的理化特性，包括光动力效应、释放动力学、体内毒性、对线虫幼虫发育的影响以及在线虫中的生物分布。该聚糖颗粒具有良好的物理化学性质，包封效率超过96%，估计保质期为3年。此外，MH-b-PS@MgPc和MH-b-PMMA@MgPc纳米载体均表现出适合PDT应用的单线态氧量子产率值，与游离MgPc的单线态氧量子产率值非常接近。药物释放行为突出了聚合物胶束核心内疏水块的性质对缓释相影响的重要性，表明MgPc与聚甲基丙烯酸甲酯的相互作用优于聚苯乙烯。此外，在低浓度下，MH-b-PS@MgPc和MH-b-PMMA@MgPc被发现对秀丽隐杆线虫无致死性，并且在口服后，它们主要积聚在肠道周围细胞中。我们的研究结果强调了含有MgPc的糖聚糖颗粒在推进第三代光敏剂用于PDT应用方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.