Telomerase Immunocytochemistry in Lymphocytes From Chronic Lymphocytic Leukemia.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Mirna Sučić, Nives Ljubić, Dubravka Županić Krmek
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引用次数: 0

Abstract

Human telomerase reverse transcriptase (hTERT) is detectable in normal progenitor cells, tumor cells, and B-cell chronic lymphocytic leukemia (B-CLL) cells. hTERT expression, in addition to other prognostic factors, is reportedly associated with a poor prognosis in B-CLL. In this study, we aimed to analyze and compare hTERT immunoexpression in B-CLL bone marrow (BM) lymphocytes and benign pleural effusion lymphocytes. Standard cytologic analysis and immunocytochemical assessment of hTERT immunoexpression were performed in BM lymphocytes from 25 patients with B-CLL and pleural effusion lymphocytes from 18 patients with pneumonia and effusion-reactive lymphocytosis. The percentages and score values of hTERT nucleus (TN)-immunopositive BM lymphocytes in patients with CLL were significantly higher than those for reactive effusion lymphocytes with no or few TN-immunopositive lymphocytes. The appearance of TN immunopositivity in CLL lymphocytes showed mainly numerous prominent or large dots, and diffused TN immunopositivity was detected; in contrast, TN-immunopositive benign effusion lymphocytes had one or few immunopositive nuclear dots. Further investigations are needed to clarify whether lymphocyte TN immunopositivity can reveal subgroups of patients with CLL with a worse prognosis and whether there is a reliable difference in TN immunopositivity between CLL and benign effusion lymphocytes.

慢性淋巴细胞白血病淋巴细胞端粒酶免疫细胞化学研究
人类端粒酶逆转录酶(hTERT)可在正常祖细胞、肿瘤细胞和b细胞慢性淋巴细胞白血病(B-CLL)细胞中检测到。据报道,除了其他预后因素外,hTERT表达与B-CLL的不良预后有关。在本研究中,我们旨在分析和比较hTERT免疫在B-CLL骨髓(BM)淋巴细胞和良性胸腔积液淋巴细胞中的表达。对25例B-CLL患者的BM淋巴细胞和18例肺炎合并积液反应性淋巴细胞增多症患者的胸腔积液淋巴细胞进行标准细胞学分析和免疫细胞化学评价hTERT免疫表达。CLL患者hTERT核(TN)免疫阳性BM淋巴细胞的百分比和评分值明显高于无或很少有TN免疫阳性淋巴细胞的反应性积液淋巴细胞。CLL淋巴细胞TN免疫阳性主要表现为大量突出或大点,可检出弥漫性TN免疫阳性;tn免疫阳性的良性积液淋巴细胞有一个或几个免疫阳性核点。淋巴细胞TN免疫阳性是否可以揭示预后较差的CLL患者亚群,以及CLL与良性积液淋巴细胞之间TN免疫阳性是否存在可靠的差异,尚需进一步研究。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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