Mutagenic impact of picric acid on chloroplast genome and a selection of biological attributes of Brassica napus L. (Brassicaceae).

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ishtiaq Ahmad, Barkat Ullah, Zahir Muhammad, Dikhnah Alshehri, Muhammad Nauman Khan, Alevcan Kaplan, Majid Iqbal, Laila A Al-Shuraym, Uthman Balgith Algopishi, Sadeq K Alhag, Meshari A Alsuwat, Diriba Dereje Olana, Muhammad Ammar Javed, Sarah Abdul Razak
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引用次数: 0

Abstract

The present study was conducted to investigate the possible mutagenic effects of picric acid on the chloroplast genome and certain biological properties of Brassica napus L. under field conditions. The experimental design for this study was a randomized complete block trial, based on 3 factors (dose, duration of priming, and three oilseed rape (cultivars). The seeds of oilseed rape cultivars (Abasin- 95, Dur e Nifa, and Nifa Gold) were exposed to five doses (0 mM, 5 mM, 10 mM, 15 mM, and 20 mM) of picric acid for different soaking times (3 h, 6 h, and 9 h) and the data on agromorphological characteristics were recorded. Control and test samples were collected and compared for genomic chloroplast studies. The results confirmed the inhibitory effect of picric acid on days to emergence, completion of germination, and percent emergence. Seeds treated with a dose of 20 mM delayed seedling emergence (6.15) compared to other treatments. In addition, the differences between seeds primed for 3 h (6.07), and 6 h (5.89) were also highly significant. Similarly, the effects of 0 mM (7.52), 5 mM (8.26) and 10 mM (8.11) doses on germination time were not significant. Comparison of the mean values with respect to germination showed that picric acid led to a reduction in the percentage emergence of the oilseed rape seedlings. A significant increase in leaf size was observed in the Nifa Gold cultivar (10.27). On the other hand, the differences in leaf size between the cultivars Dur e Nifa (9.51) and Abasin-95 (9.20) were not significant. The inhibitory effect of picric acid on the number of leaves could be due to the fact that the meristematic cells are damaged by the mutagen, leading to a reduction in the number of leaves. The Nifa Gold cultivar showed the highest value for plant height (17.53), and the highest number of siliqua (14.69) compared to other genotypes. The number of siliqua confirms that Nifa Gold is best adapted to picric acid in contrast to the other genotypes. However, the differences between the Abasin-95 cultivar (0.068) and the Nifa Gold cultivar (0.062) in terms of seed weight were not significant. The dose of 15 mM (37.72) showed the highest moisture content, followed by 10 mM (37.08), 0 mM (33.87), 5 mM (33.11) and 20 mM (32.73). The chloroplast genome of plants grown from picric acid-treated seeds was mutated. Therefore, annotation of the genome was not possible. Numerous single nucleotide substitutions (187) were detected between the control and test samples. In addition, 43 additions/deletions were observed. The present study suggests that picric acid has a mutagenic effect, which is confirmed by numerous mutations in chloroplast samples. Subsequently, the doses have stimulating effects on plant height and seed weight. Furthermore, priming can improve the emergence and certain biological characteristics of B. napus. In the present study, all parameters were investigated and the significant response of the selected genotypes was confirmed.

苦味酸对甘蓝型油菜叶绿体基因组的诱变效应及生物学特性的选择
在大田条件下,研究苦味酸对甘蓝型油菜叶绿体基因组和某些生物学特性的可能诱变效应。本研究的试验设计为随机完全区组试验,基于3个因素(剂量、启动时间和3种油菜品种)。用5种剂量(0 mM、5 mM、10 mM、15 mM和20 mM)的苦味酸浸泡3 h、6 h和9 h,记录了油菜品种(Abasin- 95、Dur e Nifa和Nifa Gold)的种子的农业形态特征。收集对照和试验样品进行基因组叶绿体研究。结果证实苦味酸对发芽天数、发芽完成率和出苗率均有抑制作用。与其他处理相比,20 mM处理种子的出苗时间延迟(6.15)。另外,处理3 h(6.07)和6 h(5.89)之间的差异也非常显著。0 mM(7.52)、5 mM(8.26)和10 mM(8.11)对发芽时间的影响也不显著。对萌发率平均值的比较表明,苦味酸降低了油菜幼苗出苗率。Nifa Gold品种(10.27)叶片大小显著增加。另一方面,杜鹃(9.51)和苦瓜-95(9.20)叶片大小差异不显著。苦味酸对叶片数量的抑制作用可能是由于诱变剂破坏了分生组织细胞,导致叶片数量减少。与其他基因型相比,Nifa Gold品种株高最高(17.53),穗数最高(14.69)。硅酸的数量证实,与其他基因型相比,Nifa Gold最适合苦味酸。而苦瓜95品种(0.068)与尼法金品种(0.062)的种子质量差异不显著。水分含量最高的是15 mM(37.72),其次是10 mM(37.08)、0 mM(33.87)、5 mM(33.11)和20 mM(32.73)。苦味酸处理后的植物叶绿体基因组发生突变。因此,基因组的注释是不可能的。在对照和测试样品之间检测到大量单核苷酸替换(187)。此外,还观察到43个增删。本研究表明苦味酸具有诱变作用,这一点已被叶绿体样品中的大量突变所证实。随后,剂量对株高和种子重有刺激作用。此外,引物还能提高甘蓝型油菜的出苗率和某些生物学特性。在本研究中,研究了所有参数,并证实了所选基因型的显著应答。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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