Cadmium sulfide nanoparticles (CdSNPs) modulate key oncogenic pathways in PA1 ovarian cancer cells: Insights from transcriptomic analysis

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2025-05-13 DOI:10.1016/j.tiv.2025.106079

Aditi Bhatnagar , Abhay Dev Tripathi , Sonali Kumari, Abha Mishra

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引用次数: 0

Abstract

Transcriptomics has become a useful tool for comparing the levels of gene expression in healthy and malignant cells, holding potential for the discovery of new cancer therapies. This study used RNA-sequencing and transcriptome analysis on the PA1 ovarian cancer cell line to examine the potential of Cadmium Sulfide Nanoparticles (CdSNPs) as a therapeutic agent. A total of 5.42 Gb of high-quality reads was estimated based on the findings of gene expression techniques, comprising 2.25 Gb of treated PA1 cells and 3.17 Gb of control cells. Of these, 1641 genes with padj<0.001 and log2 foldchange >2 were found to be significantly regulated DEGs (differentially expressed genes). Analysis of gene ontology (GO) assays demonstrates the molecular mechanism behind CdSNPs anticancer effects. GO:0006915, GO:0012501, GO:1903561, and GO:0070588 are a few significant highlights of elevated GO (enriched DEGs) that are involved in apoptotic pathways, extracellular vesicles, programmed cell death, and Ca++ signaling. KEGG analysis elucidated that up and downregulated DEGs were enriched in a few pathways: calcium signaling pathway, Apoptosis, and TNF signaling pathway. Important pathways like MAP kinase, JAK/STAT, cAMP, and folate biosynthesis, showed inhibitory effects on ovarian cancer cell proliferation. The results of this work provide insight into possible therapeutic approaches employing CdSNPs and encourage additional research using a variety of cell lines and in vivo models to improve ovarian cancer treatment.

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硫化镉纳米颗粒（CdSNPs）调节PA1卵巢癌细胞的关键致癌途径：来自转录组学分析的见解

转录组学已经成为比较健康和恶性细胞中基因表达水平的有用工具，具有发现新的癌症治疗方法的潜力。本研究通过对PA1卵巢癌细胞系的rna测序和转录组分析来研究硫化镉纳米颗粒（CdSNPs）作为治疗剂的潜力。根据基因表达技术的结果，共估计出5.42 Gb的高质量reads，其中处理过的PA1细胞为2.25 Gb，对照细胞为3.17 Gb。其中，1641个padj<；0.001和log2fold change >；2的基因被发现显著调节deg（差异表达基因）。基因本体论（GO）分析表明CdSNPs抗癌作用背后的分子机制。GO:0006915、GO:0012501、GO:1903561和GO:0070588是氧化石墨烯（富集的DEGs）升高的几个重要亮点，这些氧化石墨烯（富集的DEGs）参与凋亡途径、细胞外囊泡、程序性细胞死亡和钙离子信号传导。KEGG分析表明，上调和下调的DEGs富集于钙信号通路、凋亡信号通路和TNF信号通路。MAP激酶、JAK/STAT、cAMP、叶酸生物合成等重要通路对卵巢癌细胞增殖有抑制作用。这项工作的结果为利用CdSNPs的可能治疗方法提供了见解，并鼓励使用各种细胞系和体内模型进行进一步的研究，以改善卵巢癌的治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.