Improving sorghum growth under organic salt stress using SDS-AOT mixed micelle encapsulated indole-3-butyric acid

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-05-10 DOI:10.1016/j.molliq.2025.127754

Shachi Tiwari , Adesh Kumar , Anirudh Srivastava

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引用次数: 0

Abstract

This study investigated the interaction between indole-3-butyric acid (IBA) and mixed micelles composed of sodium dodecyl sulfate (SDS) and dioctyl sulfosuccinate sodium (AOT). Additionally, the potential application of these complexes in seed priming was explored. UV absorbance analysis revealed a hyperchromic effect and a red-shift (280–311 nm), indicating complex formation. Binding analysis demonstrated that IBA exhibited a higher affinity for mixed micelles than for individual SDS or AOT. A 1:1 stoichiometry suggested a stable interaction driven by hydrophobic forces and hydrogen bonding. Additionally, the mean occupancy (i) of IBA molecules per micelle was determined using exponential fitting. The results showed that decreasing the mole fraction of SDS (α_SDS) from 0.9 to 0.5 enhanced IBA encapsulation, improved its solubility and stability. At lower α_SDS (0.3–0.1), decreased i-values and an increase in the binding constant (K_b) suggested AOT-driven IBA interactions at the Stern layer, further enhancing stability. Docking studies predicted the highest binding affinity (−2.9 kcal/mol) for the IBA-SDS-AOT system, highlighting synergistic interactions. The IBA-AOT complex exhibited moderate binding affinity (−2.5 kcal/mol), while the IBA-SDS complex showed the weakest interaction (−2.1 kcal/mol). Seed priming with IBA-loaded mixed micelles improved sorghum germination, growth, and stress resilience under organic salt stress. Compared to IBA alone and water, priming with IBA-based mixed micelles increased emergence percentages by up to 70 % (at α_SDS 0.1). Growth parameters, including root and shoot length and biomass, improved significantly, with the highest increases observed at α_SDS 0.1 (80.11 %, 32.34 %, 76.02 %, and 33.35 %, respectively). Additionally, priming enhanced seed moisture content (25.92 % at α_SDS 0.1) and total chlorophyll levels, mitigating salt stress effects. Overall, SDS-AOT mixed micelles present an effective delivery system for hydrophobic bioactives like IBA, enhancing solubility, stability, and bioavailability. This micellar system offers a promising seed priming strategy to boost crop resilience and productivity under stress.

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吲哚-3-丁酸包封SDS-AOT胶束改善有机盐胁迫下高粱生长

研究了吲哚-3-丁酸（IBA）与十二烷基硫酸钠（SDS）和二辛基磺基琥珀酸钠（AOT）混合胶束的相互作用。此外，还探讨了这些复合物在种子引种中的潜在应用。紫外吸收分析显示出超色效应和红移（280-311 nm），表明复合物形成。结合分析表明，与单个SDS或AOT相比，IBA对混合胶束具有更高的亲和力。1:1的化学计量表明，疏水力和氢键驱动了稳定的相互作用。此外，利用指数拟合确定了每个胶团中IBA分子的平均占用率(i)。结果表明，将SDS （αSDS）的摩尔分数从0.9降低到0.5，可增强IBA的包封性，提高其溶解度和稳定性。αSDS（0.3 ~ 0.1）较低时，i值降低，结合常数（Kb）增加，表明aot驱动的IBA在Stern层相互作用，进一步增强了稳定性。对接研究预测IBA-SDS-AOT系统具有最高的结合亲和力（−2.9 kcal/mol），突出了协同作用。IBA-AOT配合物具有中等的结合亲和力（−2.5 kcal/mol），而IBA-SDS配合物的相互作用最弱（−2.1 kcal/mol）。混合胶束灌种提高了高粱在有机盐胁迫下的萌发、生长和抗逆性。与单独的IBA和水相比，IBA基混合胶束引发的羽化率提高了70% （αSDS为0.1）。根、茎长和生物量均有显著改善，在αSDS为0.1时增幅最大，分别为80.11%、32.34%、76.02%和33.35%。此外，在αSDS为0.1时，种子水分含量（25.92%）和总叶绿素含量增加，缓解了盐胁迫的影响。总之，SDS-AOT混合胶束为IBA等疏水生物活性物质提供了有效的递送系统，提高了溶解度、稳定性和生物利用度。这种胶束系统为提高作物在逆境下的抗逆性和生产力提供了一种有前途的种子启动策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.