Genetic manipulation of the betaproteobacterial genera Thauera and Aromatoleum.

Abstract

Most recombinant proteins are expressed in model host organisms like Escherichia coli. Meanwhile, a significant number of enzymes require complex activation or special cofactors not available from standard hosts. The betaproteobacteria Thauera and Aromatoleum allow access to some of these enzymes, following procedures described in this chapter. The methods described enable transformation and conjugation of vectors into these species as alternate gene expression systems which allow fundamental studies of complex recombinant proteins as well as their biotechnological application.