Broadening the diagnostic landscape ofMycobacterium tuberculosisinfection: analyzing exhaled breath.

IF 3.7 4区医学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of breath research Pub Date : 2025-05-06 DOI:10.1088/1752-7163/adcfba

Lotte W Nijman, Simona M Cristescu, Robert S Jansen

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引用次数: 0

Abstract

Mycobacterium tuberculosis(TB) is a deadly infectious agent that infects over 10 million people every year. Early detection ofM. TBinfection is essential for effective treatment and reduction of emerging drug resistance. However, current diagnostic methods are limited by lengthy procedures, invasive sampling or low sensitivity. Especially in the case of HIV co-infection, pediatric patients, EPTB and drug-resistant TB, obtaining adequate samples and detecting and treating TB is challenging. Breath analysis is an alternative tool for TB diagnosis that can potentially overcome the limitations associated with conventional techniques. Nevertheless, TB breath tests are still in their infancy. This review provides an overview of recent advances in breath analysis for TB detection. We discuss the different biomarkers found for TB detection in exhaled breath and their strengths and limitations for the disease diagnostics. We conclude that breath analysis could be a promising TB diagnosis tool, calling for standardization of breath collection and validation of data obtained with various analysis techniques to ensure both sensitivity and specificity required in practice.

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拓宽结核分枝杆菌感染的诊断领域：分析呼出气体。

结核分枝杆菌（TB）是一种致命的传染性病原体，每年感染超过1000万人。早期发现m。结核感染对于有效治疗和减少新出现的耐药性至关重要。然而，目前的诊断方法受到冗长的程序、侵入性取样或低灵敏度的限制。特别是在艾滋病毒合并感染、儿科患者、EPTB和耐药结核病的情况下，获得足够的样本以及检测和治疗结核病具有挑战性。呼吸分析是结核病诊断的一种替代工具，可以潜在地克服与传统技术相关的局限性。然而，结核病呼吸测试仍处于起步阶段。本文综述了呼气分析用于结核病检测的最新进展。我们讨论了在呼出气体中发现的用于结核病检测的不同生物标志物及其在疾病诊断中的优势和局限性。我们得出结论，呼气分析可能是一种很有前途的结核病诊断工具，需要标准化呼气收集和验证各种分析技术获得的数据，以确保实践所需的敏感性和特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM

CiteScore

7.60

自引率

21.10%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.