Pilot study of autologous multilayered fibroblast sheet transplantation for reinforcing bronchial stump healing after pulmonary lobectomy in a canine model.

IF 1.1 4区医学 Q4 CARDIAC & CARDIOVASCULAR SYSTEMS

General Thoracic and Cardiovascular Surgery Pub Date : 2025-04-15 DOI:10.1007/s11748-025-02145-y

Junichi Murakami, Toshiki Tanaka, Kenji Tani, Koji Ueno, Naohiro Yamamoto, Sota Yoshimine, Melpa Susanti Purba, Hiroshi Sunahara, Yoshinobu Hoshii, Kimikazu Hamano

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引用次数: 0

Abstract

Objective: Bronchopleural fistula (BPF) is a serious complication that can occur after lung resection. This pilot study aimed to evaluate the effectiveness of autologous multilayered fibroblast sheet transplantation in reinforcing bronchial stump healing after lung resection in a canine model.

Methods: Four beagles underwent left caudal lobe excision. Two dogs received autologous multilayered fibroblast sheet transplantation on the stapled bronchial stump, while two served as controls. Fibroblast sheets generated from autologous oral mucosal fibroblasts were optimized for growth factor secretion. Fourteen days after lobectomy, the bronchial stumps were histologically and immunohistochemically analyzed to assess connective tissue formation, blood vessel formation, and inflammation.

Results: Fibroblast sheets secreted high levels of pro-healing and pro-angiogenic factors in vitro. No adverse events or serious postoperative complications associated with the fibroblast sheet transplantation were observed. The cell sheet-transplanted group exhibited a layered structure of newly formed tissue around the bronchial stump. This was associated with enhanced blood vessel formation, as indicated by increased CD31-positive cells and high VEGF levels. The untreated control group showed a localized nodule of inflammation near the bronchial stump, which lacked evidence of blood vessel formation.

Conclusion: Autologous multilayered fibroblast sheet transplantation promoted connective tissue formation and blood vessel growth around the bronchial stump after lobectomy in a canine model. These findings suggest that fibroblast sheet transplantation is a promising therapeutic approach for preventing BPF after lung resection.

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自体多层成纤维细胞片移植增强犬肺叶切除后支气管残端愈合的初步研究。

目的：支气管胸膜瘘（BPF）是肺切除术后常见的严重并发症。本初步研究旨在评估自体多层成纤维细胞片移植在犬肺切除后支气管残端愈合中的有效性。方法：4只小猎犬行左尾叶切除。2只狗接受自体多层成纤维细胞片移植，另2只作为对照。利用自体口腔黏膜成纤维细胞制备成纤维细胞片，优化其生长因子的分泌。肺叶切除术后14天，对支气管残端进行组织学和免疫组织化学分析，以评估结缔组织形成、血管形成和炎症。结果：体外培养成纤维细胞能分泌高水平的促愈合因子和促血管生成因子。未观察到与成纤维细胞板移植相关的不良事件或严重的术后并发症。细胞片移植组在支气管残端周围呈现新形成组织的层状结构。这与血管形成增强有关，如cd31阳性细胞增加和高VEGF水平所示。未经治疗的对照组显示支气管残端附近有局部炎症结节，缺乏血管形成的证据。结论：自体多层成纤维细胞片移植促进了犬肺叶切除后支气管残端结缔组织的形成和血管的生长。这些发现表明，成纤维细胞片移植是预防肺切除术后BPF的一种有希望的治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

General Thoracic and Cardiovascular Surgery Medicine-Pulmonary and Respiratory Medicine

CiteScore

2.70

自引率

8.30%

发文量

142

期刊介绍： The General Thoracic and Cardiovascular Surgery is the official publication of The Japanese Association for Thoracic Surgery and The Japanese Association for Chest Surgery, the affiliated journal of The Japanese Society for Cardiovascular Surgery, that publishes clinical and experimental studies in fields related to thoracic and cardiovascular surgery.