Differential Expression Patterns of Bcl-2, D2-40, β-Katenin, and E-Cadherin in Thymomas: Correlation With Clinical Stages and Subtypes.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Busra Yaprak Bayrak, Aysegul U Kefeli, Cigdem Vural, Isa Cam, Ekin Y Calcali, Aykut Elicora, Huseyin F Sezer, Salih Topcu
{"title":"Differential Expression Patterns of Bcl-2, D2-40, β-Katenin, and E-Cadherin in Thymomas: Correlation With Clinical Stages and Subtypes.","authors":"Busra Yaprak Bayrak, Aysegul U Kefeli, Cigdem Vural, Isa Cam, Ekin Y Calcali, Aykut Elicora, Huseyin F Sezer, Salih Topcu","doi":"10.1097/PAI.0000000000001264","DOIUrl":null,"url":null,"abstract":"<p><p>Thymomas are rare mediastinal tumors exhibiting heterogeneous behavior. Although histologic subtypes and stages serve as prognostic factors, the molecular mechanisms of thymoma progression are unclear. Immunohistochemical markers like Bcl-2, D2-40, β-catenin, and E-cadherin offer insights into thymoma biology, but their predictive value for clinical outcomes remains uncertain. This study evaluated the expression of these markers across thymoma subtypes and stages, aiming to assess their prognostic significance. A retrospective analysis was conducted on 66 thymoma cases resected at a single center between 2005 and 2023. Immunohistochemical staining was performed to assess the expression of Bcl-2, D2-40, β-catenin, and E-cadherin. Clinicopathological characteristics were correlated with immunohistochemical findings using statistical analysis. Differential expression patterns of Bcl-2, D2-40, β-catenin, and E-cadherin were observed across thymoma subtypes and clinical stages. Bcl-2 displayed cytoplasmic positivity predominantly in type A and B thymomas, while E-cadherin showed membranous staining in type B thymomas and cytoplasmic staining in type A and AB thymomas. β-catenin demonstrated membranous staining in type B thymomas and cytoplasmic staining in type A and AB thymomas. D2-40 expression was localized to peripheral regions and invasive nests of thymomas, with higher expression in type B2 thymomas and early-stage tumors. Our findings indicate that immunohistochemical markers may provide valuable insights into thymoma biology and prognosis. Further validation in larger, multicenter cohorts is warranted to confirm the prognostic significance of these markers and their potential utility in guiding clinical management.</p>","PeriodicalId":48952,"journal":{"name":"Applied Immunohistochemistry & Molecular Morphology","volume":" ","pages":""},"PeriodicalIF":1.3000,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Immunohistochemistry & Molecular Morphology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/PAI.0000000000001264","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Thymomas are rare mediastinal tumors exhibiting heterogeneous behavior. Although histologic subtypes and stages serve as prognostic factors, the molecular mechanisms of thymoma progression are unclear. Immunohistochemical markers like Bcl-2, D2-40, β-catenin, and E-cadherin offer insights into thymoma biology, but their predictive value for clinical outcomes remains uncertain. This study evaluated the expression of these markers across thymoma subtypes and stages, aiming to assess their prognostic significance. A retrospective analysis was conducted on 66 thymoma cases resected at a single center between 2005 and 2023. Immunohistochemical staining was performed to assess the expression of Bcl-2, D2-40, β-catenin, and E-cadherin. Clinicopathological characteristics were correlated with immunohistochemical findings using statistical analysis. Differential expression patterns of Bcl-2, D2-40, β-catenin, and E-cadherin were observed across thymoma subtypes and clinical stages. Bcl-2 displayed cytoplasmic positivity predominantly in type A and B thymomas, while E-cadherin showed membranous staining in type B thymomas and cytoplasmic staining in type A and AB thymomas. β-catenin demonstrated membranous staining in type B thymomas and cytoplasmic staining in type A and AB thymomas. D2-40 expression was localized to peripheral regions and invasive nests of thymomas, with higher expression in type B2 thymomas and early-stage tumors. Our findings indicate that immunohistochemical markers may provide valuable insights into thymoma biology and prognosis. Further validation in larger, multicenter cohorts is warranted to confirm the prognostic significance of these markers and their potential utility in guiding clinical management.

胸腺瘤中Bcl-2、D2-40、β-Katenin和E-Cadherin的差异表达模式:与临床分期和亚型的相关性
胸腺瘤是一种罕见的纵隔肿瘤,表现出异质性。虽然组织学亚型和分期可作为预后因素,但胸腺瘤进展的分子机制尚不清楚。免疫组织化学标记物如Bcl-2, D2-40, β-catenin和E-cadherin提供了胸腺瘤生物学的见解,但它们对临床结果的预测价值仍不确定。本研究评估了这些标志物在胸腺瘤亚型和分期中的表达,旨在评估其预后意义。回顾性分析2005年至2023年在同一中心切除的66例胸腺瘤病例。免疫组化染色检测Bcl-2、D2-40、β-catenin、E-cadherin的表达。临床病理特征与免疫组织化学结果进行统计学分析。Bcl-2、D2-40、β-catenin和E-cadherin在不同胸腺瘤亚型和临床分期的表达差异。Bcl-2在A型和B型胸腺瘤中以细胞质阳性为主,E-cadherin在B型胸腺瘤中呈膜性染色,在A型和AB型胸腺瘤中呈细胞质染色。β-连环蛋白在B型胸腺瘤中呈膜性染色,在A型和AB型胸腺瘤中呈细胞质染色。D2-40的表达局限于胸腺瘤的外周区和浸润性巢,在B2型胸腺瘤和早期肿瘤中表达较高。我们的研究结果表明,免疫组织化学标志物可能为胸腺瘤的生物学和预后提供有价值的见解。需要在更大的多中心队列中进一步验证,以确认这些标记物的预后意义及其在指导临床管理方面的潜在效用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信