Efficiency of replacement of bone defect in rabbit jaw with germanium-doped calcium phosphate ceramics.

Abstract

Objective: Aim: To study the regenerative potential of calcium phosphate ceramics doped with germanium (CFCdGe; Ge amount 1-1,5%) compared to a deproteinized bovine material (DBM).

Patients and methods: Materials and Methods: The study was performed in vivo on 30 laboratory rabbits, which were formed with a bicortical bone defect in the mandibular angle area on both sides (diameter - 7 mm). There were 3 groups where defects were replaced by CFCdGe (group 1 [n= 10]) and DBM (group 2 [n= 10]) As a control (group 3 [n= 10]), a defect was healed under a blood clot. We studied microstructure of the CFCdGe, solubility, phase, chemical composition, and adsorption activity. The animals were euthanized on 21st, 42nd, and 90th day for taking histological samples to access trabecular width between and around granules.

Results: Results: The size of CFCdGe (Ge 1%) participles was smaller, but the porosity was larger, therefore the working concentration of Ge was chosen as 1%. At 90th day, the trabecular width around granules in group 1 (98,9±27,2 μm) was 92,78% greater than in group 2 (51,3±20,8 μm) and 22,70% greater than width of new bone trabeculae in the control group (80,6±34,2 μm) (groups 1 vs. 2: p<0,01). The trabecular width between granules in group 1 (121,0±21,0 μm) was 68,05% greater than in group 2 (72,0±13,1 μm) (p<0,01). The CFCdGe granules were surrounded by formed bone tissue with overwhelming amount of mature lamellar bone.

Conclusion: Conclusions: The CFCdGe (Ge 1%) in the experimental study on laboratory rabbits demonstrated a significantly better result than the DBM.