Xiaoxiao Qiu, Mengting Jiang, Haiyan Qu, Lin Kong, Zhihong Chai
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引用次数: 0
Abstract
Transgelin-2 (TAGLN2) is an actin-binding protein associated with tumor progression, particularly in gastric and brain tumors. However, its role in ovarian cancer metastasis is still not fully understood. This study investigated the role of TAGLN2 and its potential mechanism in ovarian cancer metastasis. TAGLN2 expression in ovarian cancer and normal tissues was assessed using Oncomine, Human Protein Atlas (HPA), and immunocytochemistry (IHC). Skov3 cells with TAGLN2 knockdown (transient knockdown of TAGLN2 of homo TAGLN2 was performed using specific small interfering RNAs), and Skov3 cells transfected with scrambled siRNAs (negative control group) were subjected to colony formation and wound healing assays to assess cell proliferation and migration in vitro. RT-PCR and western blot were used to assess TAGLN2 mRNA and protein expression; immunofluorescence staining was used to investigate the TAGLN2 impact on the cytoskeletal organization. Elevated TAGLN2 levels were observed in ovarian cancer compared with normal tissues, which was confirmed by IHC of a tissue microarray containing 65 ovarian tumor samples. TAGLN2 knockdown reduced cell proliferation and migration in vitro. Also, TAGLN2 was found to co-localize with F-actin; the knockdown of TAGLN2 impaired cytoskeletal organization, emphasizing its influence on cellular structures. In summary, TAGLN2, highly expressed in ovarian cancer, promotes migration and proliferation through cytoskeletal reorganization; thus, it may be a new therapeutic target for ovarian cancer.
Transgelin-2 (TAGLN2)是一种与肿瘤进展相关的肌动蛋白结合蛋白,特别是在胃和脑肿瘤中。然而,其在卵巢癌转移中的作用尚不完全清楚。本研究探讨TAGLN2在卵巢癌转移中的作用及其潜在机制。使用Oncomine, Human Protein Atlas (HPA)和免疫细胞化学(IHC)评估TAGLN2在卵巢癌和正常组织中的表达。TAGLN2敲低的Skov3细胞(使用特异性小干扰rna对TAGLN2同源的TAGLN2进行瞬时敲低),转染了重组sirna的Skov3细胞(阴性对照组)进行集落形成和伤口愈合实验,以评估细胞在体外的增殖和迁移。RT-PCR和western blot检测TAGLN2 mRNA和蛋白的表达;免疫荧光染色检测TAGLN2对细胞骨架组织的影响。与正常组织相比,卵巢癌组织中TAGLN2水平升高,这是通过包含65个卵巢肿瘤样本的组织微阵列的免疫组化证实的。TAGLN2敲低可降低细胞在体外的增殖和迁移。此外,TAGLN2被发现与F-actin共定位;敲低TAGLN2会破坏细胞骨架组织,强调其对细胞结构的影响。综上所述,TAGLN2在卵巢癌中高表达,通过细胞骨架重组促进迁移和增殖;因此,它可能成为卵巢癌新的治疗靶点。
期刊介绍:
Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist.
Official Journal of the International Society for Immunohistochemisty and Molecular Morphology.