Draft genome assembly of the largemouth bass (Micropterus salmoides).

Abstract

Objective: Largemouth bass (Micropterus salmoides, LMB) is an important species in aquaculture, and the annual production is rapidly increasing. Genetic and breeding studies related to LMB have promising applications, and a high-quality genome assembly is essential for interpreting genetic and sequencing data. In this study, we sequenced the genome of a male LMB using the PacBio Sequel platform, high-throughput chromosome conformation capture (Hi-C), and paired-end Illumina sequencing. Additionally, Full-length transcript sequencing was performed using isoform sequencing (Iso-Seq). Following the assembly and annotation, a draft assembly for male LMB was obtained.

Data description: This work generated PacBio data of 164.5 Gb, Hi-C data of 113.4 Gb, Illumina data of 54.7 Gb, and Iso-Seq data of 22.8 Gb. The assembly revealed that the LMB genome has a total length of 877.7 Mb, with an N50 of 37.2 Mb, comprising 23 chromosomes and 202 scaffolds. Annotation results indicated that 32.8% of the genome consists of repetitive sequences, containing 23,952 coding genes with an average gene length of 17,328 bp.