Analysis of Enzymatic Activity of Matrix Metalloproteinases by Collagen Zymography Utilizing Extracted Collagen from Bovine Muscle Tissue.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4478-2_9

Larissa A Koulicoff, Michael D Chao

引用次数: 0

Abstract

Collagen zymography is commonly used to detect the activity of collagenase matrix metalloproteinases (MMPs). This method involves incorporating collagen from various sources as a substrate into a polyacrylamide gel as a component of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The MMPs degrade the collagen in the gel under the right conditions with the presence of calcium and zinc. Here, this protocol describes the process of extracting collagen from bovine intramuscular connective tissue and incorporating it into a zymography gel for the detection of collagenase MMP activity.

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利用牛肌肉组织胶原提取，用胶原酶谱法分析基质金属蛋白酶的酶活性。

胶原酶谱法常用来检测胶原酶基质金属蛋白酶（MMPs）的活性。该方法包括将来自不同来源的胶原蛋白作为底物掺入聚丙烯酰胺凝胶中，作为十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）的组成部分。MMPs在钙和锌存在的适当条件下降解凝胶中的胶原蛋白。在这里，本方案描述了从牛肌内结缔组织中提取胶原蛋白并将其纳入酶谱凝胶以检测胶原酶MMP活性的过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.