P53 Puzzle: WWP1 and PARC Immunohistochemistry Illuminate New Pathways for Serous Ovarian Cancer.

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Elif Ozsagir, Mustafa E Ercin, Figen Celep Eyuboglu, Mehmet A Osmanagaoglu
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引用次数: 0

Abstract

High-grade serous carcinoma is categorized based on p53 mutation status. A relationship is known to exist between p53 mutations and p53 immunoexpression patterns, including overexpression, complete absence, cytoplasmic, and wild-type patterns. The ubiquitin ligases WWP1 and PARC, known to regulate p53 activation, are hypothesized to influence the pathogenesis of serous ovarian tumors. This retrospective study examined 7 low-grade serous carcinomas, 38 high-grade serous carcinomas, and 15 serous cystadenomas, with immunohistochemical analyses performed for WWP1, PARC, and p53. High-grade serous carcinoma cases were classified into wild-type, cytoplasmic, complete absence, or overexpression categories based on p53 immunohistochemistry. PARC and WWP1 expressions were compared across p53 categories and diagnoses. Results showed a statistically significant reduction in WWP1 and PARC expression in serous carcinomas, with the most pronounced loss observed in high-grade cases. Among morphologically classified high-grade carcinomas, 17 overexpression, 11 complete absence, 6 wild-type, and 4 cytoplasmic p53 cases were identified. A statistically significant relationship was found between PARC, WWP1, and p53 status. Higher expression levels of PARC and WWP1 were detected in p53 wild-type cases, whereas lower expression levels were associated with cases exhibiting p53 overexpression and complete absence. This study suggests that PARC and WWP1 play a role in the pathogenesis of high-grade serous ovarian carcinoma, potentially mediated by p53, making them promising targets for treatment and prognostic markers in serous ovarian cancer.

P53之谜:WWP1和PARC免疫组化揭示浆液性卵巢癌的新途径
高级别浆液性癌是根据p53突变状态进行分类的。已知p53突变与p53免疫表达模式之间存在关系,包括过表达、完全缺失、细胞质和野生型模式。已知调节p53激活的泛素连接酶WWP1和PARC被假设影响浆液性卵巢肿瘤的发病机制。本回顾性研究检查了7例低级别浆液性癌、38例高级别浆液性癌和15例浆液性囊腺瘤,并对WWP1、PARC和p53进行了免疫组化分析。根据p53免疫组织化学将高级别浆液性癌分为野生型、细胞质型、完全缺失型和过表达型。PARC和WWP1的表达在不同的p53类型和诊断中比较。结果显示WWP1和PARC在浆液性癌中的表达降低具有统计学意义,在高级别病例中观察到的表达减少最为明显。形态学分类的高级别癌中,p53过表达17例,完全缺失11例,野生型6例,细胞质型4例。PARC、WWP1和p53状态之间存在统计学意义上的相关性。PARC和WWP1的高表达水平在p53野生型病例中被检测到,而低表达水平与p53过表达和完全缺失的病例相关。本研究提示PARC和WWP1在高级别浆液性卵巢癌的发病机制中发挥作用,可能由p53介导,使它们成为浆液性卵巢癌治疗和预后标志物的有希望的靶点。
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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