Anoxygenic photoautotrophy driven by humus and microplastics in a photosynthetic bacterium.

IF 5.1 Q1 ECOLOGY
ISME communications Pub Date : 2025-04-18 eCollection Date: 2025-01-01 DOI:10.1093/ismeco/ycaf067
Yutong Li, Kongyuang Qu, Jianming Yang, Shuguang Wang, Zhen Yan
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Abstract

Humus and microplastics are recalcitrant organics in soils and aquatic systems, and their role in the geochemical cycling of elements remains elusive. Herein, we have identified a new mechanism by which humus and microplastics participate in anoxic carbon cycling. We demonstrated that the photoexcitation of 5-30 mg/l of humic acid or fulvic acid, two major fractions of humus, can drive CO2 fixation and enable the photoautotrophic growth of a photosynthetic bacterium, Rhodopseudomonas palustris. This process was enhanced by 10.69%-144.87% upon the addition of 100 mg/l of poly(lactic acid) or poly(ethylene terephthalate). Mechanistic investigations demonstrated that the microplastics act as sacrificial quenchers during humus photoexcitation, leading to their depolymerization. Transcriptomic analyses revealed high expression of genes encoding extracellular electron uptake pathways including extracellular cytochrome c and its oxidases in the photoautotrophic growth of R. palustris. This study expands our understanding of how humus and microplastics are involved in the biogeochemical cycling of carbon and sheds light on how they impact the CO2 dynamic fluxes in sunlit anoxic environments.

光合细菌中由腐殖质和微塑料驱动的无氧光自养。
腐殖质和微塑料是土壤和水生系统中的顽固性有机物,它们在元素地球化学循环中的作用仍然难以捉摸。在此,我们已经确定了腐殖质和微塑料参与缺氧碳循环的新机制。我们证明了5- 30mg /l腐殖酸或黄腐酸(腐殖质的两个主要部分)的光激发可以驱动CO2固定并使光合细菌Rhodopseudomonas palustris光自养生长。当添加100 mg/l聚乳酸或聚对苯二甲酸乙酯时,这一过程的增强率为10.69% ~ 144.87%。机理研究表明,微塑料在腐殖质光激发过程中作为牺牲猝灭剂,导致其解聚。转录组学分析显示,在palustris光自养生长过程中,编码细胞外电子摄取途径的基因(包括细胞外细胞色素c及其氧化酶)高表达。这项研究扩大了我们对腐殖质和微塑料如何参与碳的生物地球化学循环的理解,并揭示了它们如何影响阳光缺氧环境下的二氧化碳动态通量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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