S Horsophonphong, S Roytrakul, K Lertruangpanya, N Kitkumthorn, R Surarit
{"title":"Proteomic analysis of dental pulp from deciduous teeth in comparison to permanent teeth: an in-vitro study.","authors":"S Horsophonphong, S Roytrakul, K Lertruangpanya, N Kitkumthorn, R Surarit","doi":"10.1007/s40368-025-01043-4","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>The aims of this study were to identify proteomic profiles of dental pulp from deciduous teeth and compare the profiles of the two dentitions.</p><p><strong>Methods: </strong>Teeth that were caries-free and had normal pulp conditions were collected from twelve healthy individuals. The obtained teeth consisted of deciduous teeth (n = 6) and permanent teeth (n = 6). Proteins were extracted from pulp tissue and then analysed using liquid chromatography-tandem mass spectrometry. MaxQuant was used to identify and quantify proteins from raw mass spectrometry data of the collected deciduous and previously analysed permanent dental pulp. Differentially expressed proteins (DEPs) between the dental pulp of the two dentitions were identified by a statistical analysis conducted using Metaboanalyst with criteria P-value < 0.05 and fold change > 2.</p><p><strong>Results: </strong>A total of 3,636 proteins were identified in the dental pulp of deciduous teeth. The biological process functional classifications of these proteins were primarily concerned with cellular process, biological regulation, metabolic process and response to stimulus. Dental pulp protein profiles differed significantly between deciduous and permanent teeth, with 736 proteins being differentially expressed, the majority of which were highly expressed in the pulp of deciduous teeth. Pathway analysis indicated DEPs to be involved in tumour necrosis factor (TNF) signalling, nuclear factor kappa B signalling, and odontoclast/osteoclast differentiation.</p><p><strong>Conclusion: </strong>While the dental pulp of deciduous and permanent teeth shares some characteristics, there are also significant differences in protein expression, with the TNF signalling pathway and odontoclast/osteoclast differentiation being promoted in the dental pulp of deciduous teeth.</p>","PeriodicalId":47603,"journal":{"name":"European Archives of Paediatric Dentistry","volume":" ","pages":"941-951"},"PeriodicalIF":1.9000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12532624/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Archives of Paediatric Dentistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s40368-025-01043-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/18 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: The aims of this study were to identify proteomic profiles of dental pulp from deciduous teeth and compare the profiles of the two dentitions.
Methods: Teeth that were caries-free and had normal pulp conditions were collected from twelve healthy individuals. The obtained teeth consisted of deciduous teeth (n = 6) and permanent teeth (n = 6). Proteins were extracted from pulp tissue and then analysed using liquid chromatography-tandem mass spectrometry. MaxQuant was used to identify and quantify proteins from raw mass spectrometry data of the collected deciduous and previously analysed permanent dental pulp. Differentially expressed proteins (DEPs) between the dental pulp of the two dentitions were identified by a statistical analysis conducted using Metaboanalyst with criteria P-value < 0.05 and fold change > 2.
Results: A total of 3,636 proteins were identified in the dental pulp of deciduous teeth. The biological process functional classifications of these proteins were primarily concerned with cellular process, biological regulation, metabolic process and response to stimulus. Dental pulp protein profiles differed significantly between deciduous and permanent teeth, with 736 proteins being differentially expressed, the majority of which were highly expressed in the pulp of deciduous teeth. Pathway analysis indicated DEPs to be involved in tumour necrosis factor (TNF) signalling, nuclear factor kappa B signalling, and odontoclast/osteoclast differentiation.
Conclusion: While the dental pulp of deciduous and permanent teeth shares some characteristics, there are also significant differences in protein expression, with the TNF signalling pathway and odontoclast/osteoclast differentiation being promoted in the dental pulp of deciduous teeth.
期刊介绍：
The aim and scope of European Archives of Paediatric Dentistry (EAPD) is to promote research in all aspects of dentistry for children, including interceptive orthodontics and studies on children and young adults with special needs. The EAPD focuses on the publication and critical evaluation of clinical and basic science research related to children. The EAPD will consider clinical case series reports, followed by the relevant literature review, only where there are new and important findings of interest to Paediatric Dentistry and where details of techniques or treatment carried out and the success of such approaches are given.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
