C Butrón-Téllez Girón, I De Alba-Montero, L O Hernández-Arteaga, A Garrocho-Rangel, F Ruiz
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引用次数: 0
Abstract
Aim: The aim of the present in vitro study was to synthesize and characterize green silver nanoparticles using Larrea tridentata leaf extract as a reducing and stabilizing agent and combining the nanoparticles with a commercial fluoride varnish (Fluor Protector®) to evaluate the antibacterial activity in the production of acid and the adhesion of Streptococcus mutans and Lactobacillus acidophilus.
Methods: Silver nanoparticles were synthesized by green synthesis using Larrea tridentata leaf extract as a reducing and stabilizing agent and characterized by UV-Vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopy (TEM). Minimum inhibitory and bactericidal concentrations were determined using microdiluted plates. Antibacterial activity compared using disc diffusion. Adhesion and acidogenicity tested on deciduous teeth, divided into experimental (fluoride varnish with silver nanoparticles) and control (fluoride varnish) groups. Microorganism adhesion quantified on enamel, pH readings after 24-h incubation. Analyzed using ANOVA, Tukey's test, and Mann-Whitney U.
Results: Fluoride varnish with silver nanoparticles showed better antibacterial activity than nanoparticles and varnish alone. Fluoride varnish with silver nanoparticles was more effective compared to fluoride varnish in inhibiting the adhesion and pH decreases of Streptococcus mutans and Lactobacillus acidophilus on the sound enamel, with statistically significant differences (p < 0.001).
Conclusions: Fluoride varnish with silver nanoparticles has a good antibacterial effect for inhibiting the adhesion and production of acid being is important in the prevention and the initial stages of dental caries.
目的：本实验的目的是利用三叶草叶提取物作为还原剂和稳定剂，并将纳米颗粒与商用氟化物清漆（Fluor Protector®）结合，合成并表征绿色银纳米颗粒，以评估其在产酸过程中的抗菌活性以及对变形链球菌和嗜酸乳杆菌的粘附作用。方法：以三叶草叶提取物为还原剂和稳定剂，采用绿色合成法合成纳米银，并采用紫外可见光谱（UV-Vis）、动态光散射（DLS）和透射电子显微镜（TEM）对纳米银进行表征。用微稀释板测定最小抑菌浓度和杀菌浓度。用圆盘扩散法比较抑菌活性。在乳牙上进行附着力和致酸性试验，分为实验组（含纳米银的氟化物清漆）和对照组（含氟化物清漆）。牙釉质上微生物粘附量，培养24小时后pH值。采用方差分析、Tukey’s检验和Mann-Whitney u .分析结果：含银纳米颗粒的氟化物清漆比单独含纳米颗粒和清漆具有更好的抗菌活性。纳米银氟漆比纳米银氟漆更能有效抑制变形链球菌和嗜酸乳杆菌在健康牙釉质上的粘附和pH降低，差异有统计学意义(p)结论：纳米银氟漆具有良好的抗菌作用，可抑制牙釉质上的粘附和酸的产生，对龋病的预防和早期阶段具有重要意义。
期刊介绍：
The aim and scope of European Archives of Paediatric Dentistry (EAPD) is to promote research in all aspects of dentistry for children, including interceptive orthodontics and studies on children and young adults with special needs. The EAPD focuses on the publication and critical evaluation of clinical and basic science research related to children. The EAPD will consider clinical case series reports, followed by the relevant literature review, only where there are new and important findings of interest to Paediatric Dentistry and where details of techniques or treatment carried out and the success of such approaches are given.
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