Effect of membrane tension on pore formation induced by antimicrobial peptides and other membrane-active peptides.

IF 2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Marzuk Ahmed, Md Masum Billah, Masahito Yamazaki
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引用次数: 0

Abstract

Membrane tension plays an important role in various aspects of the dynamics and functions of cells. Here, we review recent studies of the effect of membrane tension on pore formation in lipid bilayers and pore formation induced by membrane-active peptides (MAPs) including antimicrobial peptides (AMPs). For this purpose, the micropipette aspiration method using a patch of cell membrane/lipid bilayers and a giant unilamellar vesicle (GUV)/a total cell, and the application of osmotic pressure (Π) to suspensions of large unilamellar vesicles (LUVs) have been used. However, these conventional methods have some drawbacks for the investigation of the effect of membrane tension on the actions of MAPs such as AMPs. Recently, to overcome these drawbacks, a new Π method using GUVs has been developed. Here, we focus on this Π method as a new technique for revealing the effect of membrane tension on the MAPs-induced pore formation. Firstly, we review studies of the effect of membrane tension on pore formation in lipid bilayers as determined by conventional methods. Secondly, after a brief review of studies of the effect of Π on LUVs, we describe the estimation of membrane tension in GUVs induced by Π and the Π-induced pore formation. Thirdly, after a review of the effect of membrane tension on the MAPs-induced pore formation as obtained by the conventional methods, we describe an application of the Π method to studies of the effect of membrane tension on AMP-induced pore formation. Finally, we discuss the advantages of the Π method over conventional methods and consider future perspectives.

膜张力对抗菌肽和其他膜活性肽诱导的孔形成的影响。
膜张力在细胞动力学和功能的各个方面起着重要的作用。本文综述了膜张力对脂质双分子层孔隙形成的影响以及膜活性肽(MAPs)(包括抗菌肽(amp))诱导的孔隙形成的最新研究。为此,采用微管抽吸法,利用细胞膜/脂质双分子层和巨型单层囊泡(GUV)/总细胞,并应用渗透压(Π)对大单层囊泡(LUVs)悬浮液进行抽吸。然而,这些传统的方法在研究膜张力对map(如amp)作用的影响时存在一些缺陷。最近,为了克服这些缺点,开发了一种使用guv的Π新方法。在这里,我们将重点放在Π方法上,作为一种揭示膜张力对map诱导的孔隙形成影响的新技术。首先,我们回顾了膜张力对脂质双分子层孔隙形成的影响。其次,在简要回顾了Π对luv影响的研究之后,我们描述了Π诱导的guv中膜张力的估计和Π-induced孔隙形成。第三,在回顾了膜张力对传统方法获得的map诱导孔隙形成的影响后,我们描述了Π方法在研究膜张力对amp诱导孔隙形成的影响中的应用。最后,我们讨论了Π方法相对于传统方法的优势,并考虑了未来的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physical biology
Physical biology 生物-生物物理
CiteScore
4.20
自引率
0.00%
发文量
50
审稿时长
3 months
期刊介绍: Physical Biology publishes articles in the broad interdisciplinary field bridging biology with the physical sciences and engineering. This journal focuses on research in which quantitative approaches – experimental, theoretical and modeling – lead to new insights into biological systems at all scales of space and time, and all levels of organizational complexity. Physical Biology accepts contributions from a wide range of biological sub-fields, including topics such as: molecular biophysics, including single molecule studies, protein-protein and protein-DNA interactions subcellular structures, organelle dynamics, membranes, protein assemblies, chromosome structure intracellular processes, e.g. cytoskeleton dynamics, cellular transport, cell division systems biology, e.g. signaling, gene regulation and metabolic networks cells and their microenvironment, e.g. cell mechanics and motility, chemotaxis, extracellular matrix, biofilms cell-material interactions, e.g. biointerfaces, electrical stimulation and sensing, endocytosis cell-cell interactions, cell aggregates, organoids, tissues and organs developmental dynamics, including pattern formation and morphogenesis physical and evolutionary aspects of disease, e.g. cancer progression, amyloid formation neuronal systems, including information processing by networks, memory and learning population dynamics, ecology, and evolution collective action and emergence of collective phenomena.
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