Pear flower and leaf microbiome dynamics during the naturally occurring spread of Erwinia amylovora.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-05-27 Epub Date: 2025-05-05 DOI:10.1128/msphere.00011-25
Aia Oz, Orly Mairesse, Shira Raikin, Hila Hanani, Hadar Mor, Mery Dafny Yelin, Itai Sharon
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引用次数: 0

Abstract

Erwinia amylovora is the causal pathogen of fire blight, a contagious disease that affects apple and pear trees and other members of the family Rosaceae. In this study, we investigated the community dynamics of the pear flower microbiome in an agricultural setting during the naturally occurring infection of E. amylovora. Five potential factors were considered: collection date, the flower's phenological stage, location on the tree, location within the orchard, and pear cultivar. The phenological stage and the collection date were identified as the most important factors associated with pear flower microbiome composition, while the location of the tree in the orchard and the flower's location on the tree had a marginal effect. The leaf microbiome reflected that of the abundant phenological stage on each date. The flower microbiome shifted toward E. amylovora dominating the community as time and phenological stages progressed, leading to a decreased community diversity. The E. amylovora population was represented almost exclusively by six amplicon sequence variants (ASVs) with similar proportions throughout the entire collection period. Other taxa, including Pseudomonas, Pantoea, Lactobacillus, and Sphingomonas, were represented by dozens of ASVs, and different succession patterns in their populations were observed. Some of the taxa identified include known antagonists to E. amylovora. Overall, our results suggest that flower physiology and the interaction with the environment are strongly associated with the pear flower microbiome and should be considered separately. Taxon-specific succession patterns under E. amylovora spread should be considered when choosing candidates for antagonist-based treatments for fire blight.IMPORTANCEThe spread of pathogens in plants is an important ecological phenomenon and has a significant economic impact on agriculture. Flowers serve as the entry point for E. amylovora, but members of the flower microbiome can inhibit or slow down the proliferation and penetration of the pathogen. Knowledge about leaf and flower microbiome response to the naturally occurring spread of E. amylovora is still lacking. The current study is the first to describe the Rosaceae flower microbiome dynamics during the naturally occurring infection of E. amylovora. Unlike previous studies, the study design enabled us to evaluate the contribution of five important environmental parameters to community composition. We identified different ASV succession patterns across different taxa in the flower consortia throughout the season. These results contribute to our understanding of plant microbial ecology during pathogen spread and can help improve biological treatments for fire blight.

梨花和叶片的微生物动态在自然发生的传播过程中。
淀粉状Erwinia amylovora是引起火疫病的病原体,火疫病是一种影响苹果和梨树以及蔷薇科其他成员的传染性疾病。在这项研究中,我们调查了在农业环境下梨花微生物组在自然发生的淀粉样芽孢杆菌感染期间的群落动态。考虑了五个潜在因素:采收日期、花的物候阶段、树上位置、果园内位置和梨品种。物候期和采收日期是影响梨花微生物组组成的最重要因素,而树在果园中的位置和花在树上的位置对梨花微生物组组成的影响较小。各日期叶片微生物组反映了丰富物候期的微生物组。随着时间和物候阶段的进展,花微生物群逐渐向amylovora方向转移,导致群落多样性下降。在整个收集期内,amylovora种群几乎完全由6个扩增子序列变异(amplicon sequence variants, asv)所代表,且比例相似。假单胞菌、泛菌、乳酸菌和鞘脂单胞菌等其他类群均有数十种asv,其种群演替模式各不相同。已鉴定的一些分类群包括已知的淀粉芽孢杆菌拮抗剂。总的来说,我们的研究结果表明,花的生理和与环境的相互作用与梨花微生物组密切相关,应该分开考虑。在选择以拮抗剂为基础的火疫病治疗候选植物时,应考虑淀粉孢杆菌传播下的分类群特异性演替模式。病原体在植物中的传播是一种重要的生态现象,对农业具有重大的经济影响。花是淀粉样芽孢杆菌的入口,但花微生物组的成员可以抑制或减缓病原体的增殖和渗透。关于叶片和花朵微生物组对自然发生的淀粉样芽孢杆菌传播的反应的知识仍然缺乏。目前的研究首次描述了玫瑰科花在自然发生的淀粉状芽孢杆菌感染期间的微生物组动态。与以前的研究不同,研究设计使我们能够评估五个重要环境参数对群落组成的贡献。在整个季节,我们在花群的不同分类群中发现了不同的ASV演替模式。这些结果有助于我们了解病害传播过程中的植物微生物生态,并有助于改进对火疫病的生物防治。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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