Unlocking the secrets: Exploring the connection between HPV and bladder cancer in Pakistan.

IF 2.4 3区 医学 Q3 ONCOLOGY
Hafsa Yousaf, Aneela Javed, Nuzhat Sultana, Khalid Farouk, Muhammad Usman, Gul Rehman Khan, Miriam Kathleen Gomez, Sobia Asghar, Saira Justin
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引用次数: 0

Abstract

Background: Human Papillomavirus' (HPV) clear association with anogenital carcinomas raises concerns about its role in urologic carcinomas due to anatomical proximity. This etiological link is under-researched in Pakistan, creating a critical gap in the literature.

Objectives: To decipher any probable pathological association of high-risk HPV genotypes in bladder cancer etiology.

Methods: Bladder biopsies from 63 bladder cancer patients, confirmed by H&E and IHC staining were collected with histopathological and clinical data, after informed consent. DNA was extracted and processed for HPV detection using L1 consensus primers. HPV genotyping was performed using E6-E7 specific primers for HPV16 and 18, via conventional PCR.

Result: About 15.9% (10/63) of bladder cancer cases were HPV-positive. 30% (3/10) of which were HPV16-positive, 60% (6/10) were HPV18-positive, while the remaining 1 sample (1/10) was neither positive for HPV16 nor 18 indicating a probable involvement of other high-risk genotypes. Histologically, most of the HPV-positive bladder cancers were low-grade invasive urothelial carcinoma, having a male-to-female incident ratio of 9:1 with an average age of 65 years. No significant association was found between HPV prevalence and tobacco consumption, physical activity, co-morbidities (diabetes and hypertension), and treatment effectiveness.

Conclusion: The study provides the latest epidemiological data on the prevalence and genotype distribution of HPV in bladder cancer in Pakistan. Although the frequency of HPV was not so high, nonetheless an association with bladder cancer incidence was identified as an important risk factor. Consequently, HPV remains a serious issue in low- and middle-income countries (LMICs), significantly contributing to otherwise preventable cancers.

揭开秘密:探索巴基斯坦HPV和膀胱癌之间的联系。
背景:人乳头瘤病毒(HPV)与肛门生殖器癌的明确关联引起了人们对其在泌尿系统癌中的作用的关注,因为它在解剖学上接近。巴基斯坦对这种病因学联系的研究不足,造成了文献中的重大空白。目的:探讨高危HPV基因型在膀胱癌发病中的病理关联。方法:收集63例膀胱癌患者的膀胱活检,经H&E和IHC染色证实,经知情同意后,收集组织病理学和临床资料。提取DNA并使用L1共识引物处理HPV检测。用E6-E7特异性引物对HPV16和hpv18进行常规PCR分型。结果:约15.9%(10/63)膀胱癌患者hpv阳性。其中30% (3/10)HPV16阳性,60% (6/10)hpv18阳性,其余1份(1/10)样本HPV16和hpv18均未阳性,提示可能涉及其他高危基因型。组织学上,大多数hpv阳性膀胱癌为低级别侵袭性尿路上皮癌,男女发病率为9:1,平均年龄65岁。未发现HPV患病率与烟草消费、身体活动、合并症(糖尿病和高血压)和治疗效果之间存在显著关联。结论:本研究提供了巴基斯坦膀胱癌中HPV患病率和基因型分布的最新流行病学数据。尽管HPV的发病率并不高,但与膀胱癌发病率的关联被认为是一个重要的危险因素。因此,HPV在低收入和中等收入国家(LMICs)仍然是一个严重的问题,在很大程度上导致了本可预防的癌症。
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来源期刊
CiteScore
4.80
自引率
3.70%
发文量
297
审稿时长
7.6 weeks
期刊介绍: Urologic Oncology: Seminars and Original Investigations is the official journal of the Society of Urologic Oncology. The journal publishes practical, timely, and relevant clinical and basic science research articles which address any aspect of urologic oncology. Each issue comprises original research, news and topics, survey articles providing short commentaries on other important articles in the urologic oncology literature, and reviews including an in-depth Seminar examining a specific clinical dilemma. The journal periodically publishes supplement issues devoted to areas of current interest to the urologic oncology community. Articles published are of interest to researchers and the clinicians involved in the practice of urologic oncology including urologists, oncologists, and radiologists.
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