Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies.

IF 3.7 2区生物学 Q2 MICROBIOLOGY

mSphere Pub Date : 2025-05-27 Epub Date: 2025-04-24 DOI:10.1128/msphere.00508-24

Mercedes Paz, Gonzalo Moratorio

引用次数: 0

Abstract

Recent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision. This review focuses on two powerful methodologies: deep mutational scanning and CRISPR-based genome editing, that enable comprehensive mutagenesis and detailed functional characterization of viral proteins. These approaches have significantly deepened our understanding of the molecular determinants driving viral evolution and adaptation. Furthermore, we discuss how these advances provide transformative insights for future vaccine development and therapeutic strategies.

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深度突变扫描和crispr工程病毒：进化和功能基因组学研究的工具。

合成生物学和测序技术的最新进展彻底改变了以无与伦比的精度操纵病毒基因组的能力。本文综述了两种强大的方法：深度突变扫描和基于crispr的基因组编辑，这两种方法可以实现病毒蛋白的全面突变和详细功能表征。这些方法大大加深了我们对驱动病毒进化和适应的分子决定因素的理解。此外，我们讨论了这些进展如何为未来的疫苗开发和治疗策略提供变革性的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

mSphere Immunology and Microbiology-Microbiology

CiteScore

8.50

自引率

2.10%

发文量

192

审稿时长

11 weeks

期刊介绍： mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.