Karolina Labus, Aleksandra Lambarska, Katarzyna Czyżewska, Katarzyna Szymańska
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引用次数: 0
Abstract
This publication highlights the importance of immobilisation in obtaining stable biocatalysts. It is a review of the main immobilisation methods with their advantages and disadvantages. A distinction is made between methods that require a support (adsorption, covalent binding, binding via transition metals, entrapment/encapsulation) and those that do not (cross-linking, immobilisation in a reactor volume). The main characteristics that a material should have in order to be a suitable carrier for enzymes and the most popular carrier groups are discussed. The paper also highlights the factors that should be considered when designing an immobilisation methodology and the main parameters that characterise the immobilisation process. Attention is also given to the selection of a suitable reactor solution.
期刊介绍：
The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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