Na Lu, Xiaoke Huang, Xiaolin Liang, Qin Li, Yuling Xu, Zhenfang Liu
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引用次数: 0
Abstract
Objective: To investigate the value of Lysine Demethylase 5B (KDM5B) in the prognosis and immunity of acute myeloid leukaemia (AML).
Study design: A case-control study. Place and Duration of the Study: Department of Haematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China, from 2013 to 2024.
Methodology: Bioinformatic methods were used to compare the expression of KDM5B between 135 individuals with AML and 70 individuals without AML in the TCGA database. In order to confirm the findings, bone marrow specimens underwent real-time polymerase chain reaction analysis by the Mann-Whitney U test. A comprehensive assessment was conducted to explore the interplay among KDM5B expression levels, clinical profiles, and the overall survival prognosis in AML patients. Furthermore, the underlying mechanism of KDM5B in AML pathogenesis was delved into utilising GSEA. To assess the impact of KDM5B expression on the immune landscape within tumours, the ESTIMATE and CIBERSORT computational methodologies were employed to evaluate its role in tumour immune infiltration.
Results: KDM5B expression was notably increased in AML patients and linked to a poor prognosis. KDM5B expression was correlated with NPM1 mutation in bone marrow samples. Additionally, high KDM5B expression was a poor prognostic factor for overall survival. GSEA showed that high KDM5B expression was related to the immune system. Analysis of immune infiltration revealed a correlation between elevated KDM5B expression and decreased stromal and immune scores, accompanied by altered infiltration patterns of diverse immune cell types. Furthermore, immune checkpoint markers were correlated with low KDM5B expression.
Conclusion: KDM5B is highly expressed in AML and correlates with poor prognosis. KDM5B may be involved in AML by affecting the tumour micro-environment, thus being a potential biological target in AML.
期刊介绍：
Journal of College of Physicians and Surgeons Pakistan (JCPSP), is the prestigious, peer reviewed monthly biomedical journal of the country published regularly since 1991.
Established with the primary aim of promotion and dissemination of medical research and contributed by scholars of biomedical sciences from Pakistan and abroad, it carries original research papers, , case reports, review articles, articles on medical education, commentaries, short communication, new technology, editorials and letters to the editor. It covers the core biomedical health science subjects, basic medical sciences and emerging community problems, prepared in accordance with the “Uniform requirements for submission to bio-medical journals” laid down by International Committee of Medical Journals Editors (ICMJE). All publications of JCPSP are peer reviewed by subject specialists from Pakistan and locally and abroad.
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