Vitamin D alleviates oxidative stress in varicose veins: a pilot study in obese and non-obese patients.

IF 3.7 2区 生物学 Q3 CELL BIOLOGY
Molecular and Cellular Biochemistry Pub Date : 2025-08-01 Epub Date: 2025-04-22 DOI:10.1007/s11010-025-05292-1
Sonia Rațiu, Mihaela I Mariș, Adina V Furdui-Lința, Theia Stanciu-Lelcu, Claudia Borza, Sorin Olariu, Tiberiu Bratu, Adrian Sturza, Danina M Muntean
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Abstract

Chronic venous disease and varicose veins of the lower extremities represent a widespread pathology, particularly in individuals with obesity. A high prevalence of varicose vein disease has been observed in obese patients in association with lower plasma levels of vitamin D. The present pilot study aimed to investigate the acute effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], the biologically active form of vitamin D, on oxidative stress in varicose veins obtained from both obese and non-obese patients undergoing cryostripping surgery for varicose vein ablation. Varicose venous samples treated or not with 1,25(OH)2D3 (100 nM, 12-h incubation) were analysed for reactive oxygen species (ROS) generation using the ferrous xylenol orange oxidation (FOX) assay and immunofluorescence technique. Additionally, the gene expression of endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), and inducible nitric oxide synthase (iNOS) was assessed via qPCR. We report a significant reduction in circulating 25-hydroxyvitamin D3 [25(OH)D3] levels in obese as compared to non-obese patients. Ex vivo incubation of the venous samples with 1,25(OH)2D3 resulted in: (i) significant reduction in ROS level, (ii) upregulation of eNOS and nNOS expression, and (iii) downregulation of iNOS expression in both groups of patients. Vitamin D did not exhibit a ROS scavenger effect, and the antioxidant effect is presumably mediated via its receptor whose presence was confirmed in the varicose venous samples. In conclusion, vitamin D exerts protective effects in venous pathology, which may be beneficial in acute administration prior to the surgical intervention. Large clinical trials are required to assess the optimal dosage and time/duration of administration in patients with chronic venous disease with surgical indication.

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维生素D减轻静脉曲张的氧化应激:一项针对肥胖和非肥胖患者的初步研究。
慢性静脉疾病和下肢静脉曲张是一种广泛的病理,特别是在肥胖患者中。在肥胖患者中,静脉曲张疾病的高发率与血浆维生素D水平较低有关。目前的初步研究旨在研究1,25-二羟基维生素D3 [1,25(OH)2D3](维生素D的生物活性形式)对曲张静脉氧化应激的急性影响,这些曲张静脉曲张来自接受静脉曲张消融冷冻脱皮手术的肥胖和非肥胖患者。采用二甲二酚亚铁橙色氧化(FOX)法和免疫荧光技术分析静脉曲张样品(100 nM, 12 h)的活性氧(ROS)生成情况。此外,通过qPCR检测内皮型一氧化氮合酶(eNOS)、神经元型一氧化氮合酶(nNOS)和诱导型一氧化氮合酶(iNOS)的基因表达。我们报道,与非肥胖患者相比,肥胖患者循环25-羟基维生素D3 [25(OH)D3]水平显著降低。静脉样本经1,25(OH)2D3体外孵育后:(i)两组患者的ROS水平显著降低,(ii) eNOS和nNOS表达上调,(iii) iNOS表达下调。维生素D没有表现出ROS清除作用,其抗氧化作用可能是通过其受体介导的,其存在在静脉曲张样本中得到证实。综上所述,维生素D对静脉病理具有保护作用,在手术干预前的急性给药可能是有益的。需要大型临床试验来评估有手术指征的慢性静脉疾病患者的最佳剂量和给药时间/持续时间。
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来源期刊
Molecular and Cellular Biochemistry
Molecular and Cellular Biochemistry 生物-细胞生物学
CiteScore
8.30
自引率
2.30%
发文量
293
审稿时长
1.7 months
期刊介绍: Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.
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