Transcriptomic analysis of inhibitory effects of isothiazolone antimicrobial agents on Aspergillus amstelodami ZR.

IF 1.9 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Genetics Pub Date : 2025-09-01 Epub Date: 2025-04-30 DOI:10.1007/s13353-025-00969-1

Junyu Tang, Qizhao Liang, Rui Zhang, Xiaoping Huang

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引用次数: 0

Abstract

The preservation of marine specimens requires effective methods to ensure research accuracy and ecological sustainability. However, conventional preservatives (e.g., formaldehyde) pose environmental and health risks due to their toxicity. In this study, we isolated Aspergillus amstelodami from the surface of Oreochromis mossambicus specimens and evaluated the antifungal potential of 1,2-benzoisothiazolin-3-one and Kathon. Both agents exhibited strong inhibitory effects on fungal growth, as evidenced by clear inhibition zones. Transcriptomic analysis revealed: (1) upregulation of detoxification-related genes, including cytochrome P450-mediated xenobiotic/drug metabolism, ABC transporters, and two-component systems, and (2) downregulation of ribosome biogenesis genes, impairing protein synthesis in Aspergillus amstelodami. In conclusion, this study provides novel insights into the molecular antifungal mechanisms of isothiazolone antimicrobial agents in combating contamination of marine biological specimens caused by Aspergillus amstelodami.

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异噻唑酮类抗菌剂对阿斯洛达米曲霉抑制作用的转录组学分析。

海洋标本的保护需要有效的方法来保证研究的准确性和生态的可持续性。然而，传统防腐剂（如甲醛）因其毒性而构成环境和健康风险。本研究从苔藓Oreochromis mossambicus标本表面分离出amstelodami曲霉，并对1,2-苯并异噻唑啉-3-one和卡松的抑菌活性进行了评价。两种药剂均表现出较强的抑菌作用，抑菌带明显。转录组学分析显示：(1)解毒相关基因上调，包括细胞色素p450介导的外源/药物代谢、ABC转运蛋白和双组分系统；(2)核糖体生物发生基因下调，损害了amstelodami曲霉的蛋白质合成。综上所述，本研究为异噻唑类抗菌剂对抗amstelodami曲霉污染海洋生物标本的分子抗真菌机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Genetics 生物-生物工程与应用微生物

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Applied Genetics is an international journal on genetics and genomics. It publishes peer-reviewed original papers, short communications (including case reports) and review articles focused on the research of applicative aspects of plant, human, animal and microbial genetics and genomics.