The Enhancement of Potato (Solanum Tuberosum L. Cv. Odyssey) Resistance to Bacterial Soft Rot Disease Through Transformation of the Glyphosate-Resistant Gene from Dickeya Dadanti.
IF 1.6 4区 生物学Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hossein Pasalari, Amir Roeintan, Sezai Ercisli, Danial Kahrizi, Chamran Hemmati
{"title":"The Enhancement of Potato (<i>Solanum Tuberosum</i> L. Cv. Odyssey) Resistance to Bacterial Soft Rot Disease Through Transformation of the Glyphosate-Resistant Gene from <i>Dickeya Dadanti</i>.","authors":"Hossein Pasalari, Amir Roeintan, Sezai Ercisli, Danial Kahrizi, Chamran Hemmati","doi":"10.30498/ijb.2024.461188.3934","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>An efficient protocol was developed via the <i>Agrobacterium</i>-mediated transformation method with the plasmid, p485, harboring the <i>aro</i>A gene from the bacterial species <i>Dickeya dadantii</i>, to improve resistance to potato bacterial soft rot disease. The study aimed to investigate the relationship between glyphosate application and the enhancement of potatoes' resistance to two bacterial pathogens affecting the plants.</p><p><strong>Materials and methods: </strong>An optimal concentration of 1.8 mg.L<sup>-1</sup> of glyphosate was applied to transgenic potato varieties. The leaves of the Odyssey cultivar demonstrated resistance to two pathogenic strains, <i>Pectobacterium atrosepticum</i> 21A and <i>D. dadantii</i> ENA49. Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) validation demonstrated the successful integration and heterologous expression of the <i>aro</i>A gene in the potato genome. Additionally, the transcriptional analysis revealed the expression of pathogenesis-related genes and genes associated with the potato defence response.</p><p><strong>Results: </strong>The study revealed a significant increase in the expression of pathogenesis-related genes (PR-2, PR-3, and PR-5) and defence response genes (HSR-203j and HIN1 in transgenic potato leaves after glyphosate treatment and subsequent exposure to pathogenic bacterial infection, with a particular emphasis on the upregulation of HSR-203j. A comparative analysis assessed the average expression levels of these genes in both experimental and control samples. In contrast, minimal changes in gene expression were observed in plants infected with bacteria but not treated with glyphosate.</p><p><strong>Conclusion: </strong>The study suggests that glyphosate treatment in potatoes can enhance systemic acquired resistance to bacterial pathogens by upregulating pathogenesis-related and defence response genes. This approach shows potential for addressing bacterial diseases in potatoes, including soft bacterial rot.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"22 4","pages":"e3934"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993236/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.30498/ijb.2024.461188.3934","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: An efficient protocol was developed via the Agrobacterium-mediated transformation method with the plasmid, p485, harboring the aroA gene from the bacterial species Dickeya dadantii, to improve resistance to potato bacterial soft rot disease. The study aimed to investigate the relationship between glyphosate application and the enhancement of potatoes' resistance to two bacterial pathogens affecting the plants.
Materials and methods: An optimal concentration of 1.8 mg.L-1 of glyphosate was applied to transgenic potato varieties. The leaves of the Odyssey cultivar demonstrated resistance to two pathogenic strains, Pectobacterium atrosepticum 21A and D. dadantii ENA49. Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) validation demonstrated the successful integration and heterologous expression of the aroA gene in the potato genome. Additionally, the transcriptional analysis revealed the expression of pathogenesis-related genes and genes associated with the potato defence response.
Results: The study revealed a significant increase in the expression of pathogenesis-related genes (PR-2, PR-3, and PR-5) and defence response genes (HSR-203j and HIN1 in transgenic potato leaves after glyphosate treatment and subsequent exposure to pathogenic bacterial infection, with a particular emphasis on the upregulation of HSR-203j. A comparative analysis assessed the average expression levels of these genes in both experimental and control samples. In contrast, minimal changes in gene expression were observed in plants infected with bacteria but not treated with glyphosate.
Conclusion: The study suggests that glyphosate treatment in potatoes can enhance systemic acquired resistance to bacterial pathogens by upregulating pathogenesis-related and defence response genes. This approach shows potential for addressing bacterial diseases in potatoes, including soft bacterial rot.
期刊介绍：
Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
