Immobilized metal ion affinity chromatography: waltz of metal ions and biomacromolecules.

IF 3.8 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Expert Review of Proteomics Pub Date : 2025-05-26 DOI:10.1080/14789450.2025.2492764

Rui Yan, Yan-Ming Xu, Andy T Y Lau

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引用次数: 0

Abstract

Introduction: Immobilized metal ion affinity chromatography (IMAC) is an effective method developed in the 1980s for the separation and purification of proteins. The system consists of a solid-phase matrix, a linking ligand, and a metal ion. The method is based on the ability of metal ions to bind specifically to certain specific amino acid residues of proteins, thereby selectively enriching and purifying proteins.

Areas covered: This review aims to describe current knowledge of fundamental principle of IMAC and summarize the supports, chelating ligands, and metal ions of IMAC. In addition, how IMAC technology is used in proteomics and nucleic acids research are highlighted.

Expert opinion: Over the past decades, IMAC has been extensively utilized as a predominant technique for protein enrichment in a variety of biological and medical research, such as disease diagnosis, tumor biomarker identification, protein purification, and nucleic acids research. In the future, IMAC should be integrated with other emerging proteomics technologies to promote the applications of metalloproteomes in disease diagnosis, metallodrug development, and clinical translation.

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固定化金属离子亲和层析：金属离子与生物大分子的华尔兹。

固定化金属离子亲和色谱法（IMAC）是20世纪80年代发展起来的一种有效的蛋白质分离纯化方法。该体系由固相基质、连接配体和金属离子组成。该方法是基于金属离子特异性结合蛋白质的某些特定氨基酸残基的能力，从而选择性地富集和纯化蛋白质。涵盖领域：本文综述了IMAC的基本原理，并对IMAC的载体、螯合配体和金属离子进行了综述。此外，还重点介绍了IMAC技术在蛋白质组学和DNA研究中的应用。专家意见：在过去的几十年里，IMAC作为蛋白质富集的主要技术被广泛应用于各种生物和医学研究，如疾病诊断、肿瘤生物标志物鉴定、蛋白质纯化和核酸研究。未来，IMAC应与其他蛋白质组学技术相结合，推动金属蛋白质组学在疾病诊断、金属药物开发和临床转化等方面的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Proteomics 生物-生化研究方法

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Expert Review of Proteomics (ISSN 1478-9450) seeks to collect together technologies, methods and discoveries from the field of proteomics to advance scientific understanding of the many varied roles protein expression plays in human health and disease. The journal coverage includes, but is not limited to, overviews of specific technological advances in the development of protein arrays, interaction maps, data archives and biological assays, performance of new technologies and prospects for future drug discovery. The journal adopts the unique Expert Review article format, offering a complete overview of current thinking in a key technology area, research or clinical practice, augmented by the following sections: Expert Opinion - a personal view on the most effective or promising strategies and a clear perspective of future prospects within a realistic timescale Article highlights - an executive summary cutting to the author''s most critical points.