Blood Coagulation Favors Anti-Inflammatory Immune Responses in Whole Blood.

IF 1.1 Q4 HEMATOLOGY

Hematology Reports Pub Date : 2025-04-11 DOI:10.3390/hematolrep17020019

Victor I Seledtsov, Anatoly A Pyshenko, Tatyana Ya Lyubavskaya, Irina A Seledtsova, Alexei A von Delwig

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Abstract

Background: We studied the effects of human blood coagulation on antioxidant activity and the cellular secretion of immunoregulatory molecules in vitro.

Methods: Reactive oxygen species (ROS) activity and cytokine content were determined in plasma and serum blood samples incubated with lipopolysaccharide (LPS) for 3 h or 18 h.

Results: Coagulation process significantly decreased ROS activity induced by LPS in blood samples from healthy donors. Human serum was found to have significantly higher antioxidant activity than plasma. Blood coagulation markedly reduced LPS-induced secretion of TNF-α by cells, without significantly affecting the secretion of interleukin-1 (IL-1), IL-6, IL-8, or C-reactive protein (CRP). Blood clotting led to an increase in LPS-induced release of vascular endothelial growth factor (VEGF) by blood cells. A significant increase in procalcitonin levels was also observed in serum samples.

Conclusions: Blood clotting enhances the antioxidant and anti-inflammatory functions of immunoreactive blood cells.

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凝血有利于全血的抗炎免疫反应。

背景：我们在体外研究了人血凝对机体抗氧化活性和细胞分泌免疫调节分子的影响。方法：用脂多糖（LPS）孵育3 h和18 h，测定血浆和血清中活性氧（ROS）活性和细胞因子含量。结果：凝血过程显著降低健康供者血液中LPS诱导的ROS活性。研究发现，人血清的抗氧化活性明显高于血浆。凝血可显著降低lps诱导的细胞分泌TNF-α，但对白细胞介素-1 （IL-1）、IL-6、IL-8或c反应蛋白（CRP）的分泌无明显影响。血液凝固导致lps诱导的血细胞释放血管内皮生长因子（VEGF）增加。在血清样本中也观察到降钙素原水平显著增加。结论：凝血可增强免疫反应性血细胞的抗氧化和抗炎功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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