Beneficial Effect of Rebamipide Eye Drops on Blue Light-Induced Oxidative Damage in the Ocular Surface.

IF 1.9 4区 医学 Q2 OPHTHALMOLOGY
Jingting Liu, Enying Jiang, Hyunjee Kim, Jayoung Moon, Hyeon Jeong Yoon, Kyung Chul Yoon
{"title":"Beneficial Effect of Rebamipide Eye Drops on Blue Light-Induced Oxidative Damage in the Ocular Surface.","authors":"Jingting Liu, Enying Jiang, Hyunjee Kim, Jayoung Moon, Hyeon Jeong Yoon, Kyung Chul Yoon","doi":"10.1089/jop.2024.0208","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Purpose:</i></b> We evaluated the capacity of rebamipide (REB) to alleviate corneal epithelial damage induced via blue light (BL) exposure. <b><i>Methods:</i></b> Eight-week-old C57BL/6 mice were exposed to BL (410 nm, 100 J) twice daily for 10 days. The mice were randomly divided into 5 groups: 1 untreated and 4 groups receiving BL exposure ± different topical treatments: BL exposure alone, carboxymethylcellulose, 5% <i>N</i>-acetylcysteine, and REB. Reactive oxygen species (ROS) levels were assessed, and <i>Bcl-2</i>-associated X protein (<i>BAX) protein</i> was analyzed. Apoptotic cells were detected, inflammatory cytokine levels [tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6)] were measured using enzyme-linked immunosorbent assay (ELISA), and histopathological changes in the cornea were evaluated using hematoxylin and eosin (H&E) staining. <b><i>Results:</i></b> The REB group demonstrated significantly lower BL exposure-induced ROS levels (<i>P</i> < 0.01) and <i>BAX</i> expression (<i>P</i> < 0.01) than the BL group. The number of Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive cells were lower in the REB group than in the BL group (<i>P</i> < 0.01). Furthermore, ELISA analysis revealed significantly reduced TNF-α and IL-6 levels in the REB group relative to BL group levels (<i>P</i> < 0.01). Hematoxylin and eosin staining showed preservation of corneal epithelial thickness. <b><i>Conclusions:</i></b> Rebamipide alleviated BL-induced oxidative damage to ocular surfaces by reducing ROS levels, inhibiting apoptosis, and suppressing inflammatory cytokine expression.</p>","PeriodicalId":16689,"journal":{"name":"Journal of Ocular Pharmacology and Therapeutics","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Ocular Pharmacology and Therapeutics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/jop.2024.0208","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: We evaluated the capacity of rebamipide (REB) to alleviate corneal epithelial damage induced via blue light (BL) exposure. Methods: Eight-week-old C57BL/6 mice were exposed to BL (410 nm, 100 J) twice daily for 10 days. The mice were randomly divided into 5 groups: 1 untreated and 4 groups receiving BL exposure ± different topical treatments: BL exposure alone, carboxymethylcellulose, 5% N-acetylcysteine, and REB. Reactive oxygen species (ROS) levels were assessed, and Bcl-2-associated X protein (BAX) protein was analyzed. Apoptotic cells were detected, inflammatory cytokine levels [tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6)] were measured using enzyme-linked immunosorbent assay (ELISA), and histopathological changes in the cornea were evaluated using hematoxylin and eosin (H&E) staining. Results: The REB group demonstrated significantly lower BL exposure-induced ROS levels (P < 0.01) and BAX expression (P < 0.01) than the BL group. The number of Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive cells were lower in the REB group than in the BL group (P < 0.01). Furthermore, ELISA analysis revealed significantly reduced TNF-α and IL-6 levels in the REB group relative to BL group levels (P < 0.01). Hematoxylin and eosin staining showed preservation of corneal epithelial thickness. Conclusions: Rebamipide alleviated BL-induced oxidative damage to ocular surfaces by reducing ROS levels, inhibiting apoptosis, and suppressing inflammatory cytokine expression.

利巴米胺滴眼液对蓝光引起的眼表氧化损伤的有益作用。
目的:评估利巴米胺(REB)减轻蓝光(BL)照射引起的角膜上皮损伤的能力。方法:8周龄C57BL/6小鼠每天2次暴露于BL (410 nm, 100 J),连续10 d。将小鼠随机分为5组:1组未处理,4组BL暴露±不同外用处理:BL单独暴露、羧甲基纤维素、5% n -乙酰半胱氨酸和REB。评估活性氧(ROS)水平,分析bcl -2相关X蛋白(BAX)蛋白。采用酶联免疫吸附法(ELISA)检测凋亡细胞,检测炎性细胞因子[肿瘤坏死因子-α (TNF-α)和白细胞介素-6 (IL-6)]水平,采用苏木精和伊红(H&E)染色评价角膜组织病理学变化。结果:与BL组相比,REB组小鼠体内ROS水平(P < 0.01)和BAX表达(P < 0.01)明显降低。REB组的末端脱氧核苷酸转移酶dUTP缺口末端标记(TUNEL)阳性细胞数低于BL组(P < 0.01)。此外,ELISA分析显示,相对于BL组,REB组TNF-α和IL-6水平显著降低(P < 0.01)。苏木精和伊红染色显示角膜上皮厚度保留。结论:利巴米胺通过降低ROS水平、抑制细胞凋亡、抑制炎性细胞因子表达,减轻bl诱导的眼表氧化损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.60
自引率
4.30%
发文量
72
审稿时长
1 months
期刊介绍: Journal of Ocular Pharmacology and Therapeutics is the only peer-reviewed journal that combines the fields of ophthalmology and pharmacology to enable optimal treatment and prevention of ocular diseases and disorders. The Journal delivers the latest discoveries in the pharmacokinetics and pharmacodynamics of therapeutics for the treatment of ophthalmic disorders. Journal of Ocular Pharmacology and Therapeutics coverage includes: Glaucoma Cataracts Retinal degeneration Ocular infection, trauma, and toxicology Ocular drug delivery and biotransformation Ocular pharmacotherapy/clinical trials Ocular inflammatory and immune disorders Gene and cell-based therapies Ocular metabolic disorders Ocular ischemia and blood flow Proliferative disorders of the eye Eyes on Drug Discovery - written by Gary D. Novack, PhD, featuring the latest updates on drug and device pipeline developments as well as policy/regulatory changes by the FDA.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信