The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists.

IF 5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Lipid Research Pub Date : 2025-06-01 Epub Date: 2025-04-18 DOI:10.1016/j.jlr.2025.100813
Yusuf A Hannun, Alfred H Merrill, Chiara Luberto
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引用次数: 0

Abstract

Sphingolipids and glycosphingolipids are among the most structurally diverse and complex compounds in the mammalian metabolome. They are well known to play important roles in biological architecture, cell-cell communication, and cellular regulation, and for many biological processes, multiple sphingolipids are involved. Thus, it is not surprising that untargeted genetic/transcriptomic/pharmacologic/metabolomic screens have uncovered changes in sphingolipids and sphingolipid genes/proteins while studying physiological and pathological processes. Consequently, with increasing frequency, both targeted and untargeted mass spectrometry methodologies are being used to conduct sphingolipidomic analyses. Interpretation of such large data sets and design of follow-up experiments can be daunting for investigators with limited expertise with sphingolipids (and sometimes even for someone well-versed in sphingolipidology). Therefore, this review gives an overview of essential elements of sphingolipid structure and analysis, metabolism, functions, and roles in disease and discusses some of the items to consider when interpreting lipidomics data and designing follow-up investigations.

生物活性鞘脂手册。给外行和鞘脂学家的入门书。
鞘脂和鞘脂糖是哺乳动物代谢组中结构最多样化和最复杂的化合物。鞘脂在生物结构、细胞间通讯和细胞调节中发挥着重要作用,在许多生物过程中,鞘脂涉及多种。因此,在研究生理和病理过程时,非靶向遗传/转录组学/药理学/代谢组学筛选发现鞘脂和鞘脂基因/蛋白质的变化并不奇怪。因此,随着频率的增加,靶向和非靶向质谱方法被用于进行鞘脂组学分析。解释如此庞大的数据集和后续实验的设计对于神经脂专业知识有限的研究人员(有时甚至对于精通神经脂学的人)来说是令人生畏的。因此,本文综述了鞘脂结构和分析的基本要素、代谢、功能和在疾病中的作用,并讨论了在解释脂质组学数据和设计后续调查时需要考虑的一些事项。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Lipid Research
Journal of Lipid Research 生物-生化与分子生物学
CiteScore
11.10
自引率
4.60%
发文量
146
审稿时长
41 days
期刊介绍: The Journal of Lipid Research (JLR) publishes original articles and reviews in the broadly defined area of biological lipids. We encourage the submission of manuscripts relating to lipids, including those addressing problems in biochemistry, molecular biology, structural biology, cell biology, genetics, molecular medicine, clinical medicine and metabolism. Major criteria for acceptance of articles are new insights into mechanisms of lipid function and metabolism and/or genes regulating lipid metabolism along with sound primary experimental data. Interpretation of the data is the authors’ responsibility, and speculation should be labeled as such. Manuscripts that provide new ways of purifying, identifying and quantifying lipids are invited for the Methods section of the Journal. JLR encourages contributions from investigators in all countries, but articles must be submitted in clear and concise English.
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