Timothy B Nunes, Karen L Nygard, Marc C J Courchesne, Shawn N Whitehead, Bryan S Richardson, Timothy R H Regnault
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引用次数: 0
Abstract
Fetal growth restriction is implicated in the programming of later-life neurodegeneration. We hypothesized that growth-restricted offspring would show accelerated changes to microglial white matter morphology, relative to controls. Control guinea pig sows were fed ad libitum, while maternal nutrient restriction sows received 70% of control diet switched to 90% from mid-gestation. Offspring were sacrificed at ∼26 days (neonate) or ∼110 days (adult) postpartum. Coronal brain sections from the frontal cortex were subject to IBA1 staining for microglial detection and analyzed by machine learning software. At birth, total body weight of growth-restricted offspring was reduced relative to control (p < 0.0001) with postnatal catch-up growth observed. Microglial density was reduced in the corpus callosum of control (p < 0.05) and growth-restricted (p = 0.13) adults, relative to neonates. Adults from both groups showed greater IBA1-positive area in the cingulum and periventricular white matter (p < 0.05) and increased microglial fractal dimension in the corpus callosum (p < 0.10) and periventricular white matter (p < 0.05), relative to neonates. At the time points studied, we report age-related changes in white matter microglial morphology. However, maternal nutrient restriction leading to fetal growth restriction in guinea pigs does not appear to exacerbate these white matter microglia morphological changes as a marker for later-life neurodegeneration.
胎儿生长受限与后期神经退行性变的程序化有关。我们假设,相对于对照组,生长受限的后代将显示出小胶质白质形态的加速变化。对照豚鼠母猪自由饲喂,而营养限制母猪从妊娠中期开始将70%的对照饲粮改为90%。在产后26天(新生儿)或110天(成人)处死后代。额叶皮层冠状脑切片采用IBA1染色检测小胶质细胞,并用机器学习软件进行分析。出生时,生长受限子代的总体重相对于对照组(p p p = 0.13)成人和新生儿有所降低。两组成人的扣带和脑室周围白质中iba1阳性区域均增大(p p p
期刊介绍:
Published since 1929, the Canadian Journal of Physiology and Pharmacology is a monthly journal that reports current research in all aspects of physiology, nutrition, pharmacology, and toxicology, contributed by recognized experts and scientists. It publishes symposium reviews and award lectures and occasionally dedicates entire issues or portions of issues to subjects of special interest to its international readership. The journal periodically publishes a “Made In Canada” special section that features invited review articles from internationally recognized scientists who have received some of their training in Canada.