G-Protein-coupled Estrogen Receptor 1 (GPER1) Overexpression Affects Aggressiveness of Cervical Carcinoma Cells Depending on Histological Entity.

IF 2.6 4区医学 Q2 GENETICS & HEREDITY

Cancer Genomics & Proteomics Pub Date : 2025-05-01 DOI:10.21873/cgp.20509

Lena Hambach, Julia Gallwas, Carsten Gründker

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引用次数: 0

Abstract

Background/aim: Cervical cancer (CC) is the fourth most common cancer in women worldwide. There are two main histological subtypes of CC: the more common cervical squamous cell carcinoma (CSCC) and the rarer cervical adenocarcinoma (CAC), which has a poorer prognosis. Unlike estrogen receptor (ER) α and ERβ, G-protein-coupled estrogen receptor 1 (GPER1) is recognized as a rapid mediator of cellular estrogenic action and tends to have tumor suppressive properties in CC. Since a clinical study showed that an elevated GPER1 expression is associated with a worse prognosis, we investigated the effects of stable GPER1 overexpression (GPER1-OE) on SiHa CSCC and HeLa CAC cells.

Materials and methods: SiHa CSCC and HeLa CAC cells with stable GPER1-OE were generated. GPER1-OE was tested by RT-qPCR, western blot and fluorescence-activated cell analysis (FACS). The effects of GPER1-OE on proliferation, migration, invasion, apoptosis and stem cell properties (colony and sphere formation) were then examined.

Results: Successful GPER1-OE in SiHa CSCC and HeLa CAC cells was confirmed. The cell characterization experiments showed that SiHa CSCC cells with stable GPER1-OE had faster proliferation and migration, and increased stem cell properties with larger and more numerous colonies and larger tumor spheres. In HeLa CAC cells, on the other hand, GPER1-OE resulted in slower cell proliferation, migration and invasion, reduced colony formation and tumor sphere formation. An increased rate of apoptosis was also observed.

Conclusion: GPER1-OE resulted in a more aggressive tumor behavior of SiHa CSCC cells and a less aggressive tumor behavior of HeLa CAC cells, due to a different effect of GPER1 overexpression depending on the respective histological subtypes of CC. This underlines the need for personalized medicine and a precise differentiation of subtypes in CC-related research.

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g蛋白偶联雌激素受体1 （GPER1）过表达影响宫颈癌细胞侵袭性的组织学实体

背景/目的：宫颈癌（CC）是世界范围内第四大最常见的女性癌症。CC主要有两种组织学亚型：较为常见的宫颈鳞状细胞癌（CSCC）和较为罕见的宫颈腺癌（CAC），后者预后较差。与雌激素受体(ER) α和ERβ不同，g蛋白偶联雌激素受体1 （GPER1）被认为是细胞雌激素作用的快速介质，并倾向于在CC中具有肿瘤抑制特性。由于临床研究表明GPER1表达升高与预后不良相关，我们研究了稳定的GPER1过表达（GPER1- oe）对SiHa CSCC和HeLa CAC细胞的影响。材料与方法：制备具有稳定GPER1-OE的SiHa CSCC和HeLa CAC细胞。采用RT-qPCR、western blot和荧光活化细胞分析（FACS）检测GPER1-OE。然后检测GPER1-OE对干细胞增殖、迁移、侵袭、凋亡和干细胞特性（集落和球体形成）的影响。结果：证实了GPER1-OE在SiHa和HeLa CAC细胞中的成功应用。细胞表征实验表明，具有稳定GPER1-OE的SiHa CSCC细胞具有更快的增殖和迁移速度，具有更大、更多的集落和更大的肿瘤球，干细胞特性增强。另一方面，在HeLa CAC细胞中，GPER1-OE导致细胞增殖、迁移和侵袭减慢，减少集落形成和肿瘤球形成。同时观察到细胞凋亡率升高。结论：GPER1- oe导致SiHa型CSCC细胞侵袭性更强，而HeLa型CAC细胞侵袭性更弱，这是由于GPER1过表达对CC不同组织学亚型的影响不同，这表明在CC相关研究中需要个性化治疗和精确的亚型分化。

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来源期刊

Cancer Genomics & Proteomics ONCOLOGY-GENETICS & HEREDITY

CiteScore

5.00

自引率

8.00%

发文量

期刊介绍： Cancer Genomics & Proteomics (CGP) is an international peer-reviewed journal designed to publish rapidly high quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research. In this site you may find information concerning the editorial board, editorial policy, issue contents, subscriptions, submission of manuscripts and advertising. The first issue of CGP circulated in January 2004. Cancer Genomics & Proteomics is a journal of the International Institute of Anticancer Research. From January 2013 CGP is converted to an online-only open access journal. Cancer Genomics & Proteomics supports (a) the aims and the research projects of the INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH and (b) the organization of the INTERNATIONAL CONFERENCES OF ANTICANCER RESEARCH.