Characterizing antimicrobial activity of environmental Streptomyces spp. and oral bacterial and fungal isolates from Canis familiaris and Felis catus.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-05-27 Epub Date: 2025-04-14 DOI:10.1128/msphere.00098-25
Audrey Cowen, Bonnie Yiu, Sara Fallah, Kirsten J Meyer, Emily Puumala, Yunjin Lee, Haley L Zubyk, Nicole Robbins, Justin R Nodwell, Jessie MacAlpine, Leah E Cowen
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引用次数: 0

Abstract

Antimicrobials are a pillar of modern medicine, yet our limited arsenal of antibiotics and antifungals is currently threatened by widespread drug resistance. Ongoing efforts are focused on developing strategies to identify compounds that enhance the efficacy of current antimicrobials and develop novel, resistance-evasive therapeutic strategies. In this study, we characterized microbial isolates from two distinct environments to identify those that exhibit antimicrobial activity alone and in combination with current antimicrobials: (i) oral isolates from domesticated animals and (ii) environmental Streptomyces spp. First, conditioned media prepared from bacterial and fungal oral isolates that were collected from Canis familiaris and Felis catus were screened for antibacterial and antifungal activity. Three supernatants from bacterial isolates exhibited antifungal activity against the human fungal pathogen Candida albicans in the presence of subinhibitory concentrations of fluconazole, the most widely deployed antifungal. Additionally, two bacterial isolates displayed antibacterial activity against Escherichia coli alone and in combination with the antibacterial ampicillin. Furthermore, 32 environmental isolates of confirmed and predicted Streptomyces spp. were screened for activity against C. albicans and E. coli. Cell-free media harvested from isolates WAC5038 and WAC5287 exhibited antifungal activity against Candida spp., while only the WAC5038-conditioned medium displayed antibacterial activity. Bioactivity-guided fractionation, coupled with UV/Vis absorbance spectra, suggested that the bioactive compound in WAC5287 has a similar absorbance spectrum to the antifungal class of polyenes, while the bioactive component of WAC5038 remains unknown. Overall, this work highlights a strategy to collect and screen environmental isolates for the identification of novel antimicrobials.

Importance: The emergence and spread of antimicrobial resistance presents a global health challenge. As such, researchers are focused on developing pipelines to discover novel antimicrobials. In this study, we screened two distinct collections of microbes for antimicrobial activity. First, we collected bacterial and fungal isolates from the oral cavities of domesticated dogs and cats and identified these isolates using 16S (bacteria) and ITS (fungi) sequencing. Follow-up analyses confirmed that some conditioned media from bacterial isolates had antibacterial activity against Escherichia coli and antifungal activity against Candida albicans both alone and in combination with the current antimicrobial drugs. Additionally, screening 32 predicted or confirmed Streptomyces environmental isolates for antifungal and antibacterial activity identified two isolates with antifungal activity (WAC5038 and WAC5287), with only one isolate demonstrating antibacterial activity (WAC5038). Overall, this study provides a framework to identify and characterize environmental microbes with antimicrobial activity.

环境链霉菌及家犬和猫的口腔细菌和真菌分离株的抗菌活性研究。
抗微生物药物是现代医学的支柱,但我们有限的抗生素和抗真菌药物目前正受到广泛耐药性的威胁。目前的工作重点是制定策略,以确定能够增强当前抗菌素功效的化合物,并开发新的耐药治疗策略。在这项研究中,我们对来自两种不同环境的微生物分离物进行了表征,以确定那些单独或与当前抗菌剂联合表现出抗菌活性的微生物:(i)家养动物口腔分离物和(ii)环境链霉菌。首先,从家犬和猫身上收集的细菌和真菌口腔分离物制备的条件培养基进行了抗菌和抗真菌活性筛选。三种细菌分离的上清液在亚抑制浓度的氟康唑(最广泛使用的抗真菌药物)存在下显示出对人类真菌病原体白色念珠菌的抗真菌活性。此外,两种细菌分离株对大肠杆菌单独和与抗菌氨苄西林联合表现出抗菌活性。此外,对32株已证实和预测的链霉菌进行了对白色念珠菌和大肠杆菌的活性筛选。分离物WAC5038和WAC5287的无细胞培养基对念珠菌具有抑菌活性,而只有WAC5038条件培养基具有抑菌活性。生物活性引导分离,结合紫外/可见吸收光谱,表明WAC5287中的生物活性化合物与抗真菌类多烯具有相似的吸收光谱,而WAC5038的生物活性成分尚不清楚。总的来说,这项工作强调了收集和筛选环境分离物以鉴定新型抗菌素的策略。重要性:抗菌素耐药性的出现和传播是一项全球卫生挑战。因此,研究人员正致力于开发新的抗菌剂。在这项研究中,我们筛选了两种不同的微生物群的抗菌活性。首先,我们从家养狗和猫的口腔中收集细菌和真菌分离株,并使用16S(细菌)和ITS(真菌)测序对这些分离株进行鉴定。后续分析证实,一些细菌分离的条件培养基对大肠杆菌和白色念珠菌具有抗菌活性,无论是单独使用还是与现有抗菌药物联合使用。此外,对32株预测或确认的链霉菌环境分离株进行抗真菌和抗菌活性筛选,发现2株具有抗真菌活性(WAC5038和WAC5287),只有1株具有抗菌活性(WAC5038)。总的来说,本研究为鉴定和表征具有抗菌活性的环境微生物提供了一个框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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