Complete genome sequencing and antibiofilm activity of an endophytic bacterium associated with marine sponge Hyrtios erectus collected from the Red Sea

IF 2.8 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Frontiers in Marine Science Pub Date : 2025-05-14 DOI:10.3389/fmars.2025.1588772

Mamdoh T. Jamal, Idris Abdulrahman, Ramzi H. Amran, Mohammed A. Al-Matary, Sathianeson Satheesh

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Abstract

Genome sequences of sponge bacterial endophytes will be useful for understanding the bioactive compound synthetic potential and molecular mechanisms of sponge-bacteria interactions. In this study, the complete genome of the bacterium E9 isolated from the Red Sea sponge species was sequenced and its antibiofilm activity was assessed through laboratory assay. Experiments indicated the strong antibacterial and antibiofilm activity of the extracts of bacterium E9. Complete genome sequencing reveals that genome assembly generated a single chromosome of 2123451 base pairs with a guanine-cytosine (GC) content of 32.9% with 2420 protein coding sequences and a gene/genome ratio of 83.7%. The bacterial strain was identified as Staphylococcus epidermidis based on phylogenetic analysis. A total of 9 biosynthetic gene clusters were identified in the genome using the open-source platform AntiSMASH. The observed antibacterial and antibiofilm activity of the strain E9 may be due the presence of gene clusters such as nonribosomal peptides, lasso peptides and terpenes. Overall, the whole genome analysis indicated the bacterium’s capability to adapt in diverse environments including invertebrate hosts, and bioactive compound synthesis.

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红海海绵直立人内生细菌全基因组测序及抗膜活性研究

海绵细菌内生菌的基因组序列将有助于了解生物活性化合物的合成潜力和海绵细菌相互作用的分子机制。本研究对从红海海绵中分离得到的E9细菌进行了全基因组测序，并通过实验室检测对其抗生物膜活性进行了评价。实验表明，E9菌提取物具有较强的抑菌活性和抗膜活性。全基因组测序结果显示，该基因组组装产生一条2123451个碱基对的单染色体，鸟嘌呤-胞嘧啶（GC）含量为32.9%，蛋白质编码序列为2420个，基因/基因组比为83.7%。经系统发育分析，该菌株为表皮葡萄球菌。利用开源平台AntiSMASH在基因组中共鉴定出9个生物合成基因簇。菌株E9的抗菌和抗生物膜活性可能是由于存在非核糖体肽、套索肽和萜烯等基因簇。总体而言，全基因组分析表明该细菌具有适应多种环境的能力，包括无脊椎动物宿主和生物活性化合物合成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Marine Science Agricultural and Biological Sciences-Aquatic Science

CiteScore

5.10

自引率

16.20%

发文量

2443

审稿时长

14 weeks

期刊介绍： Frontiers in Marine Science publishes rigorously peer-reviewed research that advances our understanding of all aspects of the environment, biology, ecosystem functioning and human interactions with the oceans. Field Chief Editor Carlos M. Duarte at King Abdullah University of Science and Technology Thuwal is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, policy makers and the public worldwide. With the human population predicted to reach 9 billion people by 2050, it is clear that traditional land resources will not suffice to meet the demand for food or energy, required to support high-quality livelihoods. As a result, the oceans are emerging as a source of untapped assets, with new innovative industries, such as aquaculture, marine biotechnology, marine energy and deep-sea mining growing rapidly under a new era characterized by rapid growth of a blue, ocean-based economy. The sustainability of the blue economy is closely dependent on our knowledge about how to mitigate the impacts of the multiple pressures on the ocean ecosystem associated with the increased scale and diversification of industry operations in the ocean and global human pressures on the environment. Therefore, Frontiers in Marine Science particularly welcomes the communication of research outcomes addressing ocean-based solutions for the emerging challenges, including improved forecasting and observational capacities, understanding biodiversity and ecosystem problems, locally and globally, effective management strategies to maintain ocean health, and an improved capacity to sustainably derive resources from the oceans.