Proteoform profiling of endogenous single cells from rat hippocampus at scale

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2025-05-15 DOI:10.1038/s41587-025-02669-x

Pei Su, Michael A. R. Hollas, Indira Pla, Stanislav Rubakhin, Fatma Ayaloglu Butun, Joseph B. Greer, Bryan P. Early, Ryan T. Fellers, Michael A. Caldwell, Jonathan V. Sweedler, Jared O. Kafader, Neil L. Kelleher

引用次数: 0

Abstract

We perform intact proteoform profiling of 10,809 endogenous single cells from the rat hippocampus using single-cell proteoform imaging mass spectrometry (scPiMS). scPiMS directly extracts whole proteins and demonstrates high throughput for MS-based single-cell proteomics compared with existing approaches. We develop an informatics workflow dedicated to this datatype and use it to assign neurons, astrocytes or microglia cell types according to their proteoform signatures.

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大鼠海马内源性单细胞的大规模变形分析

我们使用单细胞蛋白质形态成像质谱（scpim）对来自大鼠海马的10809个内源性单细胞进行完整的蛋白质形态分析。与现有方法相比，scpim直接提取全蛋白，具有较高的单细胞蛋白质组学通量。我们开发了一个专门用于该数据类型的信息学工作流程，并根据其蛋白质形态特征来分配神经元，星形胶质细胞或小胶质细胞类型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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