Comparative Evaluation of Antioxidant Effects of 25% Bamboo Salt and 3% Green Tea Extract on Push-out Bond Strength of AH Plus Sealer after Sodium Hypochlorite Irrigation: An In vitro Study.

Abstract

Context: Sodium hypochlorite (NaOCl), a commonly used intracanal irrigant, adversely affects the organic content of dentin, impacting the bond strength of sealer to root dentin.

Aim: The aim of the study was to compare the antioxidant effects of 25% bamboo salt and 3% green tea extract on the push-out bond strength of AH Plus sealer after NaOCl irrigation.

Subjects and methods: Forty-five single-rooted mandibular premolars were selected, decoronated, and standardized to a length of 12 mm. During the canal preparation process, ProTaper Next rotary files were used, with each step accompanied by the irrigation of 5 ml of 5.25% NaOCl. Following the instrumentation, the canals were flushed with an additional 5 ml of 5.25% NaOCl and 17% ethylenediaminetetraacetic acid. The specimens were then categorized into three groups (n = 15 each): Group 1, treated with saline; Group 2, treated with a 25% bamboo salt solution; and Group 3, treated with a 3% green tea extract solution. From the coronal and middle thirds of each root, 1.5 mm thick slices were prepared. These slices were then tested for push-out bond strength using a universal testing machine.

Statistical analysis: The data collected were subjected to one-way analysis of variance, followed by Tukey's post hoc analysis for detailed examination.

Results: The use of 5.25% NaOCl resulted in a significant reduction in the bond strength of AH Plus to dentin (P < 0.05). Among the treated groups, the samples irrigated with 3% green tea extract exhibited a notably higher push-out bond strength compared to those treated with 25% bamboo salt.

Conclusion: Within the limitations of this in vitro study, all experimental groups showed significant changes in push-out bond strength after antioxidant treatment.