A New Anti-Parietal Cell Antibody Titer Measurement Kit for Diagnosis of Autoimmune Gastritis.

IF 3 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Digestion Pub Date : 2025-04-14 DOI:10.1159/000545454

Masanori Ito, Yasuhiko Maruyama, Shuichi Terao, Kimaru Okubo, Kaori Hidaka, Tomotaka Sakamoto, Ken Haruma

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引用次数: 0

Abstract

Introduction: Recent findings revealed that autoimmune gastritis (AIG) is not rare in Japan. Therefore, the accurate diagnosis of AIG is essential in upper gastrointestinal practice. Diagnostic criteria for AIG were established in 2023; however, the conventional fluorescent antibody (FA) method for anti-parietal cell antibody (APCA) titer measurements has low accuracy and is clinically problematic.

Methods: We developed a latex agglutination (LA) method using a new human-derived antigen. Samples subjected to measurements were sera from AIG cases (127 cases, 49 males, average age 65.9 years) and control cases (129 cases, 81 males, average age 66.1 years) provided by the main facility and three affiliated facilities in Japan. APCA in whole serum was measured using the FA and LA methods.

Results: The diagnostic ability of AIG using the FA method was as follows: sensitivity of 99.2%, specificity of 41.1%, and an overall agreement rate of 69.9%, with low specificity previously being reported. On the other hand, the new LA method had good diagnostic performance with a sensitivity of 93.7%, a specificity of 95.4%, and an overall agreement rate of 94.5%.

Conclusion: We developed a new APCA measurement system that will contribute to the accurate diagnosis of AIG. The use of this measurement system in clinical practice will facilitate the diagnosis and treatment of AIG.

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一种诊断自身免疫性胃炎的新型抗壁细胞抗体滴度测定试剂盒。

最近的研究结果显示，自身免疫性胃炎（AIG）在日本并不罕见。因此，AIG的准确诊断在上消化道实践中至关重要。2023年制定了AIG诊断标准；然而，传统的荧光抗体（FA）法测定抗壁细胞抗体滴度（APCA）的准确性较低，在临床上存在问题。方法：利用一种新的人源性抗原建立胶乳凝集（LA）方法。检测样本来自日本主要机构和三个附属机构提供的AIG病例（127例，男性49例，平均年龄65.9岁）和对照病例（129例，男性81例，平均年龄66.1岁）的血清。采用FA法和LA法测定全血清APCA。结果：FA法诊断AIG的敏感性为99.2%，特异性为41.1%，总体符合率为69.9%，特异性较低，既往有报道。另一方面，新的LA方法具有良好的诊断性能，灵敏度为93.7%，特异性为95.4%，总体符合率为94.5%。结论：我们开发了一种新的APCA测量系统，有助于AIG的准确诊断。在临床实践中使用该测量系统将有助于对AIG的诊断和治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Digestion 医学-胃肠肝病学

CiteScore

7.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Digestion'' concentrates on clinical research reports: in addition to editorials and reviews, the journal features sections on Stomach/Esophagus, Bowel, Neuro-Gastroenterology, Liver/Bile, Pancreas, Metabolism/Nutrition and Gastrointestinal Oncology. Papers cover physiology in humans, metabolic studies and clinical work on the etiology, diagnosis, and therapy of human diseases. It is thus especially cut out for gastroenterologists employed in hospitals and outpatient units. Moreover, the journal''s coverage of studies on the metabolism and effects of therapeutic drugs carries considerable value for clinicians and investigators beyond the immediate field of gastroenterology.