RNA Sequencing Identifies Novel Signaling Pathways and Potential Drug Target Genes Induced by FOSL1 in Glioma Progression and Stemness.

IF 3.4 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Biologics : Targets & Therapy Pub Date : 2025-04-05 eCollection Date: 2025-01-01 DOI:10.2147/BTT.S509774
Shanchun Guo, Rajveer Sidhu, Vanajothi Ramar, Alyssa A Guo, Guangdi Wang, Mingli Liu
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引用次数: 0

Abstract

Background: Glioblastoma is a highly aggressive brain tumor, and the transition from the proneural to mesenchymal subtype is associated with more aggressive and therapy-resistant features. However, the signaling pathways and genes involved in this transition remain largely undefined.

Methods: We utilized patient-derived xenograft (PDX) samples of glioblastoma, specifically PDX-L14, which exhibit both negative and overexpressed FOSL1 expression. mRNA expression profiles were assessed by RNA sequencing in these samples, followed by gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and Gene Set Enrichment Analysis (GSEA). Validation of the hub genes was performed using qPCR and immunohistochemistry assays.

Results: Differentially expressed genes (DEGs) between FOSL1 overexpression groups were predominantly involved in ferroptosis, immune response, angiogenesis, vascular mimicry, autophagy, epithelial-mesenchymal transition (EMT), cancer cell stemness, temozolomide (TMZ) resistance, and NF-κB signaling. Downregulated DEGs were associated with TMZ resistance, glioma proliferation, RNA processing, and Wnt/β-catenin signaling. Key enrichment pathways, including NF-κB, Want, and BMP, are all critical for maintaining glioma stemness. FOSL1 was found to regulate RNA processing and ubiquitination. Notably, 8 upregulated (ITGA5, SDC1, PHLDB2, TNFRSF8, ADAM8, TLR7, STEAP3, and POU3F2) and 4 downregulated (IFIT1, FBXO16, ARL3, and BEX1) genes were identified, with implications for glioblastoma prognosis.

Conclusion: This transcriptome investigation emphasizes the diverse functions of FOSL1 in different biological processes and signaling networks during the shift from proneural to mesenchymal state in glioblastoma.

RNA测序鉴定由FOSL1诱导的神经胶质瘤进展和干细胞的新信号通路和潜在药物靶基因。
背景:胶质母细胞瘤是一种高度侵袭性的脑肿瘤,从前膜亚型向间充质亚型的转变与更具侵袭性和治疗耐药的特征相关。然而,参与这一转变的信号通路和基因在很大程度上仍未明确。方法:我们利用胶质母细胞瘤患者来源的异种移植(PDX)样本,特别是PDX- l14,其FOSL1表达为阴性和过表达。通过RNA测序评估这些样本的mRNA表达谱,随后进行基因本体(GO)分析、京都基因与基因组百科全书(KEGG)途径分析和基因集富集分析(GSEA)。采用qPCR和免疫组织化学方法对枢纽基因进行验证。结果:FOSL1过表达组间差异表达基因(DEGs)主要参与铁凋亡、免疫应答、血管生成、血管模拟、自噬、上皮-间质转化(EMT)、癌细胞干细胞性、替莫唑胺(TMZ)耐药和NF-κB信号转导。下调的DEGs与TMZ耐药、胶质瘤增殖、RNA加工和Wnt/β-catenin信号传导有关。关键的富集途径,包括NF-κB、Want和BMP,都是维持胶质瘤干性的关键。发现FOSL1调节RNA加工和泛素化。值得注意的是,有8个基因上调(ITGA5、SDC1、PHLDB2、TNFRSF8、ADAM8、TLR7、STEAP3和POU3F2), 4个基因下调(IFIT1、FBXO16、ARL3和BEX1),这与胶质母细胞瘤的预后有关。结论:这项转录组研究强调了FOSL1在胶质母细胞瘤从前膜状态向间质状态转变的不同生物学过程和信号网络中的不同功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biologics : Targets & Therapy
Biologics : Targets & Therapy MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
8.30
自引率
0.00%
发文量
22
审稿时长
16 weeks
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