PLAROsomes as a modified liposomes delivery system for Mimosa pudica L. extract: Augmenting anticancer potential against prostate and skin cancer cell lines.

IF 1 Q4 PHARMACOLOGY & PHARMACY

Annales pharmaceutiques francaises Pub Date : 2025-04-24 DOI:10.1016/j.pharma.2025.04.004

Vaishali K Gaikwad, Ravindra B Laware, Nitin Mohire, Somnath Devidas Bhinge

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引用次数: 0

Abstract

Objective: PLAROsomes, a modified liposomal drug carrier system, were developed to overcome the major drawback of drug leakage in conventional liposomes. This study aimed to encapsulate Mimosa pudica L. extract (MIPA) within PLAROsomes to enhance anticancer efficacy against prostate (PC-3) and skin (B16F10) cancer cell lines.

Method: PLAROsomes were formulated using the thin-film hydration technique and characterized through infrared (IR) and ultraviolet (UV) spectroscopy, thermal analysis (TGA and DSC), particle size distribution, zeta potential measurement, and morphological assessment via SEM and TEM. Key formulation parameters, including encapsulation efficiency (%EE), drug loading capacity (%DLC), and drug release (%DR), were optimized for stability and efficacy. Cytotoxicity was evaluated using the MTT assay. Additionally, MIPA-loaded PLAROsomes were prepared without 2-methyl-resorcinol to assess its role in preventing drug leakage.

Results: The optimized MIPA-loaded PLAROsomes had a particle size of 193.2±41.6nm, confirmed by TEM at 139±15nm. They exhibited higher encapsulation efficiency (83.45±0.45%) and drug loading (9.85%) compared to formulations without 2-methyl-resorcinol (74.56±0.65% EE and 8.80% DLC), indicating its stabilizing effect. Drug release followed the Korsmeyer-Peppas model, demonstrating a sustained profile. At 100μg/mL, MIPA-loaded PLAROsomes significantly reduced cell viability (52.22±1.54% in B16F10 and 45.57±0.80% in PC-3), outperforming the free extract.

Conclusion: MIPA-loaded PLAROsomes exhibit enhanced anticancer potential and could serve as an effective targeted therapy, warranting further clinical investigation.

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PLAROsomes作为改良的含羞草提取物脂质体递送系统：增强对前列腺和皮肤癌细胞系的抗癌潜力。

目的：为克服常规脂质体药物漏药的缺点，研制了一种改良的脂质体药物载体体系PLAROsomes。本研究旨在将含羞草提取物（Mimosa pudica L. extract， MIPA）包埋在PLAROsomes内，以提高其对前列腺（PC-3）和皮肤（B16F10）癌细胞的抗癌作用。方法：采用薄膜水化技术制备PLAROsomes，并通过红外（IR）和紫外（UV）光谱、热分析（TGA和DSC）、粒度分布、zeta电位测量以及SEM和TEM形态学分析对其进行表征。对包封率（%EE）、载药量（%DLC）、释药量（%DR）等关键处方参数进行了稳定性和有效性优化。采用MTT法评价细胞毒性。此外，制备了不含2-甲基间苯二酚的mipa负载PLAROsomes，以评估其在防止药物泄漏中的作用。结果：优化后的负载mipa的PLAROsomes的粒径为193.2±41.6nm， TEM在139±15nm处证实。与不含2-甲基间苯二酚的制剂（EE为74.56±0.65%，DLC为8.80%）相比，其包封效率（83.45±0.45%）和载药量（9.85%）更高，表明其具有稳定作用。药物释放遵循Korsmeyer-Peppas模型，显示出持续的特征。在100μg/mL浓度下，负载mipa的PLAROsomes显著降低细胞活力（B16F10为52.22±1.54%，PC-3为45.57±0.80%），优于游离提取物。结论：负载mipa的plarosome具有增强的抗癌潜力，可作为有效的靶向治疗，值得进一步的临床研究。

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来源期刊

Annales pharmaceutiques francaises PHARMACOLOGY & PHARMACY-

CiteScore

1.70

自引率

7.70%

发文量

期刊介绍： This journal proposes a scientific information validated and indexed to be informed about the last research works in all the domains interesting the pharmacy. The original works, general reviews, the focusing, the brief notes, subjected by the best academics and the professionals, propose a synthetic approach of the last progress accomplished in the concerned sectors. The thematic Sessions and the – life of the Academy – resume the communications which, presented in front of the national Academy of pharmacy, are in the heart of the current events.