tRF5-22-SerGCT-1 protects the heart against myocardial injury by targeting MSK1.

IF 3 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2025-05-01 Epub Date: 2025-04-23 DOI:10.1080/17501911.2025.2495544

Fanji Meng, Hemanyun Bai, Kangling Ke, Lingyan Fang, Haitao Huang, Xiao Liang, Weiyan Li, Xiongwen Chen, Can Chen

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引用次数: 0

Abstract

Aim: This study aims to explore the expression profiles and potential functions of tsRNAs in MI.

Methods: Using a mouse model of MI induced by coronary artery ligation, we used smallRNA array to obtain tsRNAs expression profiles. Reverse transcription quantitative polymerase chain reaction(RT-qPCR), Western Blot, tRF5-22-SerGCT-1 mimics and inhibitors, cell proliferation and apoptosis detection, luciferase reporter assay, and bioinformatics analysis were employed to screen differentially expressed tsRNAs and identify the functions of tsRNAs after MI.

Results: A total of 175 significantly different tsRNAs (FC > 1.5, p < 0.05) were identified in MI mice, including 98 upregulated and 77 downregulated tsRNAs. Bioinformatics and target gene prediction revealed that two differentially expressed tsRNAs (5'tiRNA-34-GlnCTG-4, tRF5-22-SerGCT-1) may be involved in processes like autophagy and apoptosis, as well as in key signaling pathways such as MAPK and autophagy. Further investigation of tRF5-22-SerGCT-1 revealed that its overexpression or inhibition in vitro affected MSK1 levels and cardiomyocytes apoptosis following oxygen-glucose deprivation, providing a protective effect. Dual-luciferase assays confirmed that tRF5-22-SerGCT-1 targets MSK1.

Conclusion: We found differentially expressed tsRNAs in MI. In addition, our research showed first that tRF5-22-SerGCT-1 might be involved in the MAPK pathways by targeting the MSK1, modulating apoptosis.

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tRF5-22-SerGCT-1通过靶向MSK1保护心脏免受心肌损伤。

目的：探讨tsRNAs在心肌梗死中的表达谱及其潜在功能。方法：以冠状动脉结扎致心肌梗死小鼠为模型，采用小rna阵列技术获取tsRNAs的表达谱。采用逆转录定量聚合酶链反应（RT-qPCR）、Western Blot、tRF5-22-SerGCT-1模拟物和抑制物、细胞增殖和凋亡检测、荧光素酶报告基因检测、生物信息学分析等方法筛选差异表达的tsRNAs，并鉴定mi后tsRNAs的功能。结果：共有175种差异显著的tsRNAs （FC > 1.5, p）。此外，我们的研究首次发现tRF5-22-SerGCT-1可能通过靶向MSK1参与MAPK通路，调节细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.