Soluble alpha klotho-impact of biological variables and reference intervals for adults.

IF 5.3 1区医学 Q1 ENDOCRINOLOGY & METABOLISM

European Journal of Endocrinology Pub Date : 2025-04-30 DOI:10.1093/ejendo/lvaf093

Júnia Ribeiro de Oliveira Longo Schweizer, Katharina Schilbach, Michael Haenelt, Anica Gagliardo, Annette Peters, Barbara Thorand, Sylvère Störmann, Jochen Schopohl, Martin Reincke, Michael Lauseker, Martin Bidlingmaier

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引用次数: 0

Abstract

Objective: Concentrations of soluble alpha klotho (sαKL) are higher in active acromegaly compared with healthy controls. However, reference intervals based on large population-based samples are lacking, and the impact of many biological variables is unclear.

Design: Cross-sectional study.

Methods: We measured sαKL concentrations in samples from an adult population (20-89 years, 435 males, 455 females). Associations with sex, age, body mass index, waist-hip-ratio, estimated glomerular filtration rate (eGFR), IGF-I and IGFBP 3, glucose-, lipid-, calcium- and liver-metabolism, fasting, and estrogen status were analyzed. Reference intervals were calculated using LMS quantile regression with a Box-Cox transformation to normality. We also analyzed sαKL in patients with non-functioning pituitary adenoma (NFPA, n = 18) and prolactinoma (n = 65).

Results: Across all ages, sαKL concentrations (pg/mL, median [interquartile ranges]) were slightly, but significantly higher in females compared with males (678 (537-859) vs. 651 (537-812), P = .01), suggesting an impact of estrogens. SαKL exhibited a weak negative correlation with age, and positive correlations with eGFR and IGF-I (P < .001 for both). Correlations to other biological factors including glucose, liver and calcium metabolism and duration of fasting were negligible (P > .05 for all). Compared with sαKL, IGF-I more often was correlated significantly to other biological variables. SαKL was not different in patients with NFPA, but slightly higher in patients with prolactinoma (P < .05).

Conclusion: Our findings suggest sαKL is a stable GH-sensitive biomarker that may be less impacted by biological variables compared with IGF-I and IGFBP 3. Our reference intervals will facilitate the potential use of sαKL in GH-related diseases.

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可溶性α - klotho -成人生物变量和参考区间的影响。

目的：活动期肢端肥大症患者可溶性α - klotho （s - α kl）浓度高于健康对照组。然而，缺乏基于大规模人口样本的参考区间，并且许多生物变量的影响尚不清楚。设计：横断面研究。方法：我们测量了成年人群（20-89岁，男性435人，女性455人）样本中的s - α kl浓度。分析了与性别、年龄、体重指数、腰臀比、估计肾小球滤过率（eGFR）、IGF-I和IGFBP 3、葡萄糖、脂质、钙和肝脏代谢、禁食和雌激素状态的关系。使用LMS分位数回归计算参考区间，并进行Box-Cox正态转换。我们还分析了无功能垂体腺瘤（NFPA, n=18）和泌乳素瘤（n=65）患者的s - α kl。结果：在所有年龄段，女性的s - α kl浓度（pg/mL，中位数（IQR））略高于男性（678（537-859）比651 (537-812),p=0.01），提示雌激素的影响。s - α kl与年龄呈弱负相关，与eGFR、IGF-I呈正相关（均p0.05）。与s - α kl相比，IGF-I更常与其他生物学变量显著相关。结论：与IGF-I和IGFBP 3相比，s - α kl是一种稳定的gh敏感生物标志物，受生物学变量的影响较小。我们的参考区间将促进s - α kl在gh相关疾病中的潜在应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Endocrinology 医学-内分泌学与代谢

CiteScore

9.80

自引率

3.40%

发文量

354

审稿时长

1 months

期刊介绍： European Journal of Endocrinology is the official journal of the European Society of Endocrinology. Its predecessor journal is Acta Endocrinologica. The journal publishes high-quality original clinical and translational research papers and reviews in paediatric and adult endocrinology, as well as clinical practice guidelines, position statements and debates. Case reports will only be considered if they represent exceptional insights or advances in clinical endocrinology. Topics covered include, but are not limited to, Adrenal and Steroid, Bone and Mineral Metabolism, Hormones and Cancer, Pituitary and Hypothalamus, Thyroid and Reproduction. In the field of Diabetes, Obesity and Metabolism we welcome manuscripts addressing endocrine mechanisms of disease and its complications, management of obesity/diabetes in the context of other endocrine conditions, or aspects of complex disease management. Reports may encompass natural history studies, mechanistic studies, or clinical trials. Equal consideration is given to all manuscripts in English from any country.