Quercetin-calcium hydroxide scaffolds modulate dental pulp stem cell response in vitro under a simulated inflammatory environment

IF 7.1 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2025-04-26 DOI:10.1111/iej.14243

Caroline Anselmi, Igor Paulino Mendes Soares, Sarah Chang, Lais M. Cardoso, Ana Beatriz Gomes de Carvalho, Renan Dal-Fabbro, Carlos Alberto de Souza Costa, Marco C. Bottino, Josimeri Hebling

{"title":"Quercetin-calcium hydroxide scaffolds modulate dental pulp stem cell response in vitro under a simulated inflammatory environment","authors":"Caroline Anselmi, Igor Paulino Mendes Soares, Sarah Chang, Lais M. Cardoso, Ana Beatriz Gomes de Carvalho, Renan Dal-Fabbro, Carlos Alberto de Souza Costa, Marco C. Bottino, Josimeri Hebling","doi":"10.1111/iej.14243","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>Tissue engineering can be applied to dentine regeneration, stimulating tissue repair by promoting mesenchymal cell migration and differentiation into odontoblast-like cells while modulating inflammation. This study aimed to investigate the effect of quercetin (QU) and calcium hydroxide (CH) incorporated into polycaprolactone (PCL)/polyethylene oxide (PEO) scaffolds on the differentiation of dental pulp stem cells (DPSCs) in a simulated inflammatory environment in vitro.</p>\n </section>\n \n <section>\n \n <h3> Methodology</h3>\n \n <p>Dental pulp stem cells (DPSCs) were cultured and treated with different concentrations of quercetin (QU) to assess cell viability, mineralized matrix production and responses under inflammatory stimuli. Reactive oxygen and nitrogen species, as well as TNF-α synthesis, were quantified using fluorescence and ELISA methods. Scaffolds of PCL/PEO with calcium hydroxide and QU were fabricated via electrospinning, characterized and analysed for cell adhesion, viability, inflammatory and mineralisation-related genes in an artificial pulp chamber model. Statistical analysis was performed using <span>anova</span>, Kruskal–Wallis and confidence intervals with a significance level of 5%.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Polycaprolactone/polyethylene oxide scaffolds incorporated with CH and QU showed cytocompatibility and support for DPSC differentiation at concentrations of up to 5 M diluted in the culture medium. After 14 days of treatment, the scaffolds upregulated <i>ALPL</i> gene expression under the inflammatory stimulus, with no differences between the control group and the nonincorporated scaffold. The expression of osteocalcin (<i>OCN</i>) and dentine sialophosphoprotein (<i>DSPP</i>) genes was significantly upregulated for the scaffold-treated group when stimulated with LPS.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Incorporating QU and CH into PCL/PEO scaffolds modulated the inflammatory-related response and upregulated mineralisation-related genes of LPS-challenged dental pulp stem cells.</p>\n </section>\n </div>","PeriodicalId":13724,"journal":{"name":"International endodontic journal","volume":"58 7","pages":"1073-1090"},"PeriodicalIF":7.1000,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International endodontic journal","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/iej.14243","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Aim

Tissue engineering can be applied to dentine regeneration, stimulating tissue repair by promoting mesenchymal cell migration and differentiation into odontoblast-like cells while modulating inflammation. This study aimed to investigate the effect of quercetin (QU) and calcium hydroxide (CH) incorporated into polycaprolactone (PCL)/polyethylene oxide (PEO) scaffolds on the differentiation of dental pulp stem cells (DPSCs) in a simulated inflammatory environment in vitro.

Methodology

Dental pulp stem cells (DPSCs) were cultured and treated with different concentrations of quercetin (QU) to assess cell viability, mineralized matrix production and responses under inflammatory stimuli. Reactive oxygen and nitrogen species, as well as TNF-α synthesis, were quantified using fluorescence and ELISA methods. Scaffolds of PCL/PEO with calcium hydroxide and QU were fabricated via electrospinning, characterized and analysed for cell adhesion, viability, inflammatory and mineralisation-related genes in an artificial pulp chamber model. Statistical analysis was performed using anova, Kruskal–Wallis and confidence intervals with a significance level of 5%.

Results

Polycaprolactone/polyethylene oxide scaffolds incorporated with CH and QU showed cytocompatibility and support for DPSC differentiation at concentrations of up to 5 M diluted in the culture medium. After 14 days of treatment, the scaffolds upregulated ALPL gene expression under the inflammatory stimulus, with no differences between the control group and the nonincorporated scaffold. The expression of osteocalcin (OCN) and dentine sialophosphoprotein (DSPP) genes was significantly upregulated for the scaffold-treated group when stimulated with LPS.

Conclusions

Incorporating QU and CH into PCL/PEO scaffolds modulated the inflammatory-related response and upregulated mineralisation-related genes of LPS-challenged dental pulp stem cells.

查看原文本刊更多论文

槲皮素-氢氧化钙支架在体外模拟炎症环境下对牙髓干细胞反应的调节。

目的：将组织工程技术应用于牙本质再生，通过促进间充质细胞向成牙细胞样细胞的迁移和分化，同时调节炎症，刺激组织修复。本研究旨在探讨槲皮素（QU）和氢氧化钙（CH）掺入聚己内酯(PCL)/聚氧聚乙烯（PEO）支架对体外模拟炎症环境下牙髓干细胞（DPSCs）分化的影响。方法：培养牙髓干细胞（DPSCs），并用不同浓度的槲皮素（QU）处理，以评估细胞活力、矿化基质的产生和炎症刺激下的反应。采用荧光法和ELISA法定量测定活性氧和活性氮种类以及TNF-α的合成。采用静电纺丝法制备了PCL/PEO氢氧化钙和QU支架，并在人工牙髓室模型中对细胞粘附、活力、炎症和矿化相关基因进行了表征和分析。采用方差分析、Kruskal-Wallis和显著性水平为5%的置信区间进行统计分析。结果：加入CH和QU的聚己内酯/聚氧化物支架在培养基中稀释至5 M时显示出细胞相容性和支持DPSC分化的能力。治疗14天后，支架在炎症刺激下上调ALPL基因表达，对照组与未掺入支架之间无差异。LPS刺激支架组骨钙素（OCN）和牙本质唾液磷蛋白（DSPP）基因表达显著上调。结论：在PCL/PEO支架中加入QU和CH可调节lps刺激牙髓干细胞的炎症相关反应和矿化相关基因的上调。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.