Genetic variant in LncRNA-NKILA associated with gastric cancer susceptibility and the binding with miR-6731-5p.

IF 3.9 3区医学 Q1 PATHOLOGY

Expert Review of Molecular Diagnostics Pub Date : 2025-05-02 DOI:10.1080/14737159.2025.2499898

Qiuyu Sun, Ye Zhang, Qian Yang, Xiaolin Chen, Yiran Wang, Yuwei Hou, Yaodong Zhang, Kaijuan Wang

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引用次数: 0

Abstract

Background: This study aims to assess the association between lncRNA-NKILA single nucleotide polymorphisms (SNPs) and susceptibility to gastric cancer in the Chinese Han population.

Research design and methods: Four functional SNPs (rs911157, rs16981280, rs2273534, and rs957313) were validated using bioinformatics analysis and genotyped in 490 patients and 490 controls. A case-control study was conducted to analyze the association between NKILA SNPs and gastric cancer risk. qRT-PCR was conducted to detect the NKILA expression in plasma of different rs911157 and rs16981280 genotypes. The effect of rs16981280 C>T mutation on the binding ability of NKILA and miR-6731-5p was verified by dual-luciferase experiment.

Results: In this study, rs911157 CT genotype (OR:1.72, 95%CI:1.20-2.69) was associated with an increased risk of gastric cancer, whereas rs16981280 CG (OR:0.64, 95%CI:0.48-0.85) and GG genotypes (OR:0.56, 95%CI:0.36-0.87) were associated with a reduced risk. The population attributable risk percentage for rs911157 T-carriers was below 10%, while for the rs16981280 CC genotype was approximately 15%. Rs911157 C carried a binding site with miR-6731-5p while T allele might cause the target loss.

Conclusions: In summary, NKILA polymorphism might be related to the susceptibility of gastric cancer. NKILA rs911157 C/T genotype probably affects the function of gastric cancer cells by modulating the interactions with of miR-6731-5p.

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LncRNA-NKILA基因变异与胃癌易感性及与miR-6731-5p的结合

背景：本研究旨在评估中国汉族人群lncRNA-NKILA单核苷酸多态性（snp）与胃癌易感性的关系。研究设计与方法：通过生物信息学分析验证4个功能snp (rs911157、rs16981280、rs2273534和rs957313)，并对490例患者和490例对照进行基因分型。我们进行了一项病例对照研究，分析NKILA snp与胃癌风险的关系。采用qRT-PCR检测不同rs911157和rs16981280基因型血浆中NKILA的表达。通过双荧光素酶实验验证rs16981280 C>T突变对NKILA与miR-6731-5p结合能力的影响。结果：在本研究中，rs911157 CT基因型（OR:1.72, 95%CI:1.20-2.69）与胃癌风险增加相关，而rs16981280 CG基因型（OR:0.64, 95%CI:0.48-0.85）和GG基因型（OR:0.56, 95%CI:0.36-0.87）与风险降低相关。rs911157 t -携带者的人群归因风险百分比低于10%，而rs16981280 CC基因型的人群归因风险百分比约为15%。Rs911157 C携带miR-6731-5p结合位点，T等位基因可能导致靶基因丢失。结论：综上所述，NKILA多态性可能与胃癌易感性有关。NKILA rs911157 C/T基因型可能通过调节与miR-6731-5p的相互作用影响胃癌细胞的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Molecular Diagnostics 医学-病理学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Expert Review of Molecular Diagnostics (ISSN 1473-7159) publishes expert reviews of the latest advancements in the field of molecular diagnostics including the detection and monitoring of the molecular causes of disease that are being translated into groundbreaking diagnostic and prognostic technologies to be used in the clinical diagnostic setting. Each issue of Expert Review of Molecular Diagnostics contains leading reviews on current and emerging topics relating to molecular diagnostics, subject to a rigorous peer review process; editorials discussing contentious issues in the field; diagnostic profiles featuring independent, expert evaluations of diagnostic tests; meeting reports of recent molecular diagnostics conferences and key paper evaluations featuring assessments of significant, recently published articles from specialists in molecular diagnostic therapy. Expert Review of Molecular Diagnostics provides the forum for reporting the critical advances being made in this ever-expanding field, as well as the major challenges ahead in their clinical implementation. The journal delivers this information in concise, at-a-glance article formats: invaluable to a time-constrained community.