Regulation of the microRNA profiles related to Myh7 and Myh6 in myocardial ischemia through proanthocyanidins and different intensity exercise training.

Abstract

Objective: Myocardial ischemia (MI) and circulatory arrest are associated with unfavorable cardiovascular outcomes. This study aims to investigate the effects of proanthocyanidins (PC) and regular exercise with various intensity training protocols (low, moderate, and high) on cardiac protection in a rat model of MI induced by isoproterenol.

Materials and methods: Based on bioinformatics, a pool of microRNAs and mRNAs was assessed according to significant differential expression in MI condition. Further, the networks of hub genes and mRNA-microRNAs were constructed. After 14 weeks of low, moderate, and high-intensity interval training and oral administration of 300 mg/kg of PC, MI was established in the rats by injecting isoproterenol. The real-time qPCR assessed the relative expressions.

Results: Based on the in-silico analysis, Fn1 (fibronectin-1) and Myh7 (myosin heavy chain 7) are potentially druggable cut points to reduce cardiac tissue damage. High-intensity interval training (HIIT) and consumption of PC modified the relative expression of Myh6 (myosin heavy chain 6), Myh7, and Nf1. Moreover, High-intensity interval training and PC regulated the mir92a-3p, mir181a-5p, mir29a-3p, and mir133a-3p.

Conclusion: Here, the data indicated that the HIIT protocol could have an effective strategy compared to low-intensity interval training (LIIT) and moderate-intensity interval training protocols (MIIT). Furthermore, HIIT and PC might have protective effects on the MI condition.