Untargeted metabolic analysis using LC-Q-TOF-MS and toxicity assessment of Eryngium foetidum in zebrafish embryos.

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Acta Pharmaceutica Pub Date : 2025-04-10 Print Date: 2025-03-01 DOI:10.2478/acph-2025-0008
Romario Vázquez-Cancino, Sergio Rodríguez-Morales, Nelly Del Carmen Jiménez-Pérez, Omar Aristeo Peña-Morán, Litzia Cerón-Romero, Irma Sánchez-Lombardo, Alam Yair-Hidalgo, Nancy Romero Ceronio, Cuauhtémoc Alvarado-Sánchez, Oswaldo Hernández-Abreu
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引用次数: 0

Abstract

Toxicological studies of edible plant species are important to determine the safety of their consumption. Eryngium foetidum is an edible plant used in some countries for seasoning food and as a natural remedy in folk medicine. Despite this species' gastronomic and medicinal properties, the chemical composition and toxicity have been unclear. The objective of our investigation was to determine the toxic potential of E. foetidum in the zebrafish embryo model and identify the potential compounds involved in its toxicity by electrospray ionization liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry. Acute exposure of zebrafish embryos to n-hexane extract produced higher toxicity than the other extracts in a time- and concentration-dependent fashion (coagulated embryo). A 96-h median lethal concentration (LC 50) of 2.63 µg mL-1 (CI 95 % 0.58-28.5) was calculated by probit analysis. Caudal fin hypertrophy, head, yolk sac edema, caudal region, or somite malformations were observed. Secondary metabolites such as terpenes, polyphenols, and fatty acids were identified in the n-hexane extract. Also, pollutants such as diglycidyl resorcinol ether, diisopropyl adipate, and lauryl sulfate were found in the n-hexane extract. Our study revealed that chemical pollutants could be associated with the embryonic toxicity of the n-hexane extract of E. foetidum.

LC-Q-TOF-MS法分析斑马鱼胚胎中胎氧的非靶向代谢及毒性评价。
可食用植物的毒理学研究对确定其食用安全性具有重要意义。羊角草是一种可食用的植物,在一些国家用作调味食品和作为民间医学的天然药物。尽管该物种具有美食和药用特性,但其化学成分和毒性尚不清楚。本研究的目的是通过电喷雾电离液相色谱-四极杆飞行时间质谱联用技术,确定胎儿草在斑马鱼胚胎模型中的潜在毒性,并鉴定其毒性可能涉及的化合物。斑马鱼胚胎急性暴露于正己烷提取物产生的毒性比其他提取物在时间和浓度依赖性(凝固胚胎)。probit分析法计算96 h的中位致死浓度(lc50)为2.63µg mL-1 (CI 95% 0.58 ~ 28.5)。尾鳍肥大、头、卵黄囊水肿、尾鳍区或部分畸形。在正己烷提取物中鉴定出次生代谢物,如萜烯、多酚和脂肪酸。此外,在正己烷提取物中还发现了污染物,如间苯二酚二缩水甘油酯醚、己二酸二异丙酯和硫酸十二烷基。我们的研究表明,化学污染物可能与胎草正己烷提取物的胚胎毒性有关。
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来源期刊
Acta Pharmaceutica
Acta Pharmaceutica PHARMACOLOGY & PHARMACY-
CiteScore
5.20
自引率
3.60%
发文量
20
审稿时长
>12 weeks
期刊介绍: AP is an international, multidisciplinary journal devoted to pharmaceutical and allied sciences and contains articles predominantly on core biomedical and health subjects. The aim of AP is to increase the impact of pharmaceutical research in academia, industry and laboratories. With strong emphasis on quality and originality, AP publishes reports from the discovery of a drug up to clinical practice. Topics covered are: analytics, biochemistry, biopharmaceutics, biotechnology, cell biology, cell cultures, clinical pharmacy, drug design, drug delivery, drug disposition, drug stability, gene technology, medicine (including diagnostics and therapy), medicinal chemistry, metabolism, molecular modeling, pharmacology (clinical and animal), peptide and protein chemistry, pharmacognosy, pharmacoepidemiology, pharmacoeconomics, pharmacodynamics and pharmacokinetics, protein design, radiopharmaceuticals, and toxicology.
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