Experimental visceral leishmaniasis: immunopathology and histology.

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cellular and molecular biology Pub Date : 2025-04-26 DOI:10.14715/cmb/2025.71.4.11

Rabia Ali Aboud, Safaa A Fadil, Ahmed Akil Khudhair Al-Daoody, Amani M Jasim

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Abstract

This study aimed to assess the immunological and histopathological effects of Leishmania donovani infection in mice, and the impact of pentostam treatment. L. donovani promastigotes were cultured in Nicolle-Novy-McNeal (NNN) medium. Thirty mice were divided into three groups of ten: a negative control group given saline, a positive control group infected with promastigotes, and a treatment group infected with promastigotes and treated with pentostam. The mice were treated daily for 21 days. Blood samples were collected after 7, 14, and 21 days to measure serum levels of IL-1. After 21 days, the mice were euthanized, and their livers and spleens were collected for histopathological analysis. The results showed a significant decrease in IL-1 levels in the infected group compared to the control group, while IL-1 levels increased slightly in the treated group. Histopathological analysis revealed pathological changes in the liver and spleen of infected mice, which were reduced in the treated group. The study concluded that L. donovani infection leads to a decrease in IL-1 production and causes pathological damage to the liver and spleen, and that pentostam treatment is effective in mitigating these effects.

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实验性内脏利什曼病：免疫病理和组织学。

本研究旨在评估小鼠感染多诺瓦利什曼原虫的免疫学和组织病理学影响，以及戊妥胺治疗的影响。在Nicolle-Novy-McNeal （NNN）培养基中培养L. donovani promastigotes。将30只小鼠分为3组，每组10只：阴性对照组给予生理盐水，阳性对照组感染原鞭毛菌，治疗组感染原鞭毛菌并给予戊氧胺治疗。小鼠每天治疗21天。第7、14、21天采集血样，测定血清IL-1水平。21 d后对小鼠实施安乐死，取肝、脾进行组织病理学分析。结果显示，与对照组相比，感染组的IL-1水平显著下降，而治疗组的IL-1水平略有上升。组织病理学分析显示，感染小鼠的肝脏和脾脏发生病理改变，治疗组的肝脏和脾脏病变减少。本研究认为，多诺瓦氏乳杆菌感染可导致IL-1生成减少，并对肝脏和脾脏造成病理性损害，戊妥胺治疗可有效减轻这些影响。

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来源期刊

Cellular and molecular biology 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

331

期刊介绍： Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.