Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats.

IF 1.9 Q3 CHEMISTRY, MEDICINAL

Avicenna Journal of Phytomedicine Pub Date : 2025-01-01 DOI:10.22038/AJP.2024.24819

Mahsan Alizadeh, Sina Moshtagh, Shahabaddin Abdolalizadeh Amir, Mostafa Jeddi, Sepehr Tahmasebzadeh, Ghazal Radman, Amin Bagheri, Yasin Bagheri, Nazanin Shahabinejad

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Abstract

Objective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.

Materials and methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights were conducted. Reproductive potential was evaluated by ELISA for testosterone, LH, and FSH levels, as well as sperm characteristics. Oxidative stress levels were assessed through MDA measurements and antioxidant enzyme activities. Additionally, apoptotic and signaling pathway proteins were analyzed by measuring cleaved caspase-3 and Nrf-2 levels in the testes using western blot analysis.

Results: GO mitigated depressive behaviors and reduced serum corticosterone levels compared to the CRS-control group. GO increased testosterone, LH, and FSH levels and improved sperm parameters. GO supplementation reduced MDA levels and enhanced antioxidant enzyme activities compared to the CRS-control group. The analysis showed that GO down-regulated cleaved caspase-3 levels and up-regulated Nrf-2 protein levels in the testes of CRS rats compared to the CRS-control group.

Conclusion: The administration of GO treatment can contribute to the recovery from male reproductive complications by regulating oxidative stress and apoptotic pathways.

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γ -米甲醇对慢性限制性应激大鼠雄性生殖功能的抗氧化和抗凋亡作用。

目的：研究氧化石墨烯对CRS暴露大鼠抑郁行为和生殖参数的影响。材料与方法：将大鼠分为假手术组、CRS-control组和CRS-GO组。使用SPT进行行为评估，并测量身体和睾丸重量。通过ELISA检测睾丸激素、黄体生成素和卵泡刺激素水平以及精子特征，评估生殖潜能。通过测定丙二醛和抗氧化酶活性来评估氧化应激水平。此外，通过western blot分析睾丸中cleaved caspase-3和Nrf-2的水平，分析凋亡和信号通路蛋白。结果：与crs对照组相比，氧化石墨烯减轻了抑郁行为，降低了血清皮质酮水平。氧化石墨烯增加睾丸激素、黄体生成素和卵泡刺激素水平，改善精子参数。与crs对照组相比，氧化石墨烯补充剂降低了MDA水平，增强了抗氧化酶活性。分析显示，与CRS对照组相比，氧化石墨烯可下调CRS大鼠睾丸中cleaved caspase-3水平，上调Nrf-2蛋白水平。结论：氧化石墨烯治疗可通过调节氧化应激和细胞凋亡通路促进男性生殖并发症的恢复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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