Pedunculoside regulates the differentiation of neural stem cells into neurons via the PI3K/AKT/GSK-3β pathway

IF 3 3区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of pharmacological sciences Pub Date : 2025-05-02 DOI:10.1016/j.jphs.2025.05.002

Ruichen Jiang , Yuming Wang , Yanzhu Shen , Jiancheng Tang , Xiangsheng Tang , Haoning Ma , Ping Yi

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Abstract

The aging population has increased neurodegenerative diseases, yet current therapies mostly relieve symptoms rather than halt disease progression. Neural stem cells (NSCs) are crucial in nervous system development and repair, and research on their proliferation and differentiation regulation is vital. This study aimed to explore the effect of pedunculoside (PE) on primary NSCs and its mechanism. NSCs from pregnant rats (E13 - E14) were cultured and studied using CCK - 8, EDU incorporation, immunofluorescence, Western blot, and molecular docking. Results showed PE significantly promoted NSC proliferation at 10 μM and 20 μM dose - dependently. It also enhanced neuronal differentiation, with increased TUJ - 1 and decreased GFAP. Molecular docking and Western blot revealed PE binds to PI3K, activates the PI3K/AKT/GSK-3β pathway, and promotes protein phosphorylation. The PI3K inhibitor experiment confirmed PE's effect on NSC differentiation is mediated by this pathway. This study provides evidence for PE's role in NSC research and advances nervous system disease treatment research.

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pedculloside通过PI3K/AKT/GSK-3β通路调控神经干细胞向神经元的分化

人口老龄化增加了神经退行性疾病，但目前的治疗大多是缓解症状，而不是停止疾病进展。神经干细胞（Neural stem cells, NSCs）在神经系统发育和修复中起着至关重要的作用，对其增殖和分化调控的研究具有重要意义。本研究旨在探讨pedculloside （PE）对原发性NSCs的影响及其机制。采用CCK - 8、EDU掺入、免疫荧光、Western blot、分子对接等方法对妊娠大鼠（E13 ~ E14）的NSCs进行培养和研究。结果显示，PE在10 μM和20 μM剂量下显著促进NSC增殖。它还能促进神经元分化，增加TUJ - 1，降低GFAP。分子对接和Western blot结果显示，PE与PI3K结合，激活PI3K/AKT/GSK-3β通路，促进蛋白磷酸化。PI3K抑制剂实验证实PE对NSC分化的影响是通过这一途径介导的。本研究为PE在NSC研究中的作用提供了证据，并推动了神经系统疾病的治疗研究。

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来源期刊

Journal of pharmacological sciences 医学-药学

CiteScore

6.20

自引率

2.90%

发文量

104

审稿时长

31 days

期刊介绍： Journal of Pharmacological Sciences (JPS) is an international open access journal intended for the advancement of pharmacological sciences in the world. The Journal welcomes submissions in all fields of experimental and clinical pharmacology, including neuroscience, and biochemical, cellular, and molecular pharmacology for publication as Reviews, Full Papers or Short Communications. Short Communications are short research article intended to provide novel and exciting pharmacological findings. Manuscripts concerning descriptive case reports, pharmacokinetic and pharmacodynamic studies without pharmacological mechanism and dose-response determinations are not acceptable and will be rejected without peer review. The ethnopharmacological studies are also out of the scope of this journal. Furthermore, JPS does not publish work on the actions of biological extracts unknown chemical composition.