Chemical coupling of biological magnetosomes with amine-functionalized therapeutic antibody conjugates

IF 2.8 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sandy M. Payan, Lucía Gandarias, Romain Avellan, Damien Faivre, Sandra Prévéral
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引用次数: 0

Abstract

BACKGROUND

Immunotherapy, particularly in cancer treatment, can be enhanced using antibody–drug or antibody–radionuclide conjugates. These conjugates disrupt cell signaling and induce cell death, requiring the targeted antigen to be highly expressed on tumor cells to avoid damage to healthy tissues. One promising strategy to improve delivery of antibody conjugates is the use of magnetosomes, biological magnetic nanoparticles, which can be guided to tumor sites using magnetic fields. However, most antibody–drug or antibody–radionuclide conjugates are functionalized using the antibody amine groups of lysine residues on the heavy chains. Therefore, these amine groups are no longer available to bind the antibodies to the magnetosomes.

RESULTS

Here, we explore an alternative approach to bind amine-functionalized antibodies to magnetosomes. Using sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate as a crosslinker, the antibodies are chemically attached to the magnetosome membrane via thiol groups through antibody partial reduction. Our results demonstrate that this chemical process preserves the integrity and functionality of both magnetosomes and antibodies. Moreover, we prove that the produced magnetosome–antibody conjugates are stable under various in vivo-like conditions.

CONCLUSION

This coupling method offers significant advantages, enabling the coupling of therapeutic molecules to antibodies combined with the magnetic properties of magnetosomes. This new strategy aims to improve cancer therapy through targeted delivery and rapid accumulation at tumor sites. © 2025 Society of Chemical Industry (SCI).

生物磁小体与胺功能化治疗性抗体偶联物的化学偶联
免疫治疗,特别是在癌症治疗中,可以使用抗体-药物或抗体-放射性核素缀合物来增强免疫治疗。这些偶联物破坏细胞信号传导并诱导细胞死亡,需要靶向抗原在肿瘤细胞上高度表达以避免损伤健康组织。一种有希望改善抗体偶联物递送的策略是使用磁小体,生物磁性纳米颗粒,它可以通过磁场引导到肿瘤部位。然而,大多数抗体-药物或抗体-放射性核素偶联物是利用重链上赖氨酸残基的抗体胺基功能化的。因此,这些胺基不再可用于将抗体与磁小体结合。在这里,我们探索了一种将胺功能化抗体与磁小体结合的替代方法。抗体以磺基琥珀酰亚胺基-4-(n -马来酰亚胺甲基)环己烷-1-羧酸盐为交联剂,通过抗体部分还原,通过巯基化学方式附着在磁小体膜上。我们的研究结果表明,这一化学过程保留了磁小体和抗体的完整性和功能。此外,我们证明了所产生的磁小体抗体偶联物在各种体内样条件下是稳定的。结论该偶联方法具有明显的优势,可以将治疗分子偶联到结合磁小体磁性的抗体上。这种新策略旨在通过靶向递送和肿瘤部位的快速积累来改善癌症治疗。©2025化学工业学会(SCI)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.00
自引率
5.90%
发文量
268
审稿时长
1.7 months
期刊介绍: Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.
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